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CD74 Antibody

Purified Mouse Monoclonal Antibody

  • E - CD74 Antibody AO1835a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - CD74 Antibody AO1835a
    Figure 1: Western blot analysis using CD74 mAb against human CD74 recombinant protein. (Expected MW is 37.6 kDa)
  • WB - CD74 Antibody AO1835a
    Figure 2: Western blot analysis using CD74 mAb against HEK293 (1) and CD74 (AA: 1-106)-hIgGFc transfected HEK293 (2) cell lysate.
  • WB - CD74 Antibody AO1835a
    Figure 3: Western blot analysis using CD74 mouse mAb against Raji cell lysate.
  • IF - CD74 Antibody AO1835a
    Figure 3: Immunofluorescence analysis of Hela cells using CD74 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
  • FC - CD74 Antibody AO1835a
    Figure 4: Flow cytometric analysis of Jurkat cells using CD74 mouse mAb (green) and negative control (red).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P04233
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 2D1B3
Isotype IgG1
Calculated MW 33.5kDa
Description The protein encoded by this gene associates with class II major histocompatibility complex (MHC) and is an important chaperone that regulates antigen presentation for immune response. It also serves as cell surface receptor for the cytokine macrophage migration inhibitory factor (MIF) which, when bound to the encoded protein, initiates survival pathways and cell proliferation. This protein also interacts with amyloid precursor protein (APP) and suppresses the production of amyloid beta (Abeta). Multiple alternatively spliced transcript variants encoding different isoforms have been identified.
Immunogen Purified recombinant fragment of human CD74 (AA: 1-106) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 972
Other Names HLA class II histocompatibility antigen gamma chain, HLA-DR antigens-associated invariant chain, Ia antigen-associated invariant chain, Ii, p33, CD74, CD74, DHLAG
Dilution E~~1/10000
WB~~1/500 - 1/2000
IF~~1/200 - 1/1000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCD74 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CD74
Synonyms DHLAG
Function Plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to the endosomal/lysosomal system where the antigen processing and binding of antigenic peptides to MHC class II takes place. Serves as cell surface receptor for the cytokine MIF.
Cellular Location Cell membrane; Single-pass type II membrane protein. Endoplasmic reticulum membrane. Golgi apparatus, trans-Golgi network. Endosome Lysosome. Note=Transits through a number of intracellular compartments in the endocytic pathway. It can either undergo proteolysis or reach the cell membrane
Research Areas
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The protein encoded by this gene is a transcriptional activator. It can bind the consensus DNA sequence 5'-DHWATTGAYTWWD-3' on a variety of gene promoters such as those of HNF3B and TTR(By similarity). ;


1. Tumour Biol. 2012 Dec;33(6):2273-7. 2. World J Gastroenterol. 2012 May 14;18(18):2253-61.

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$ 385.00
Cat# AO1835a
(40 western blots)
Availability: 7-10 days
Bulk Size
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