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ONECUT3 Antibody

Purified Mouse Monoclonal Antibody

     
  • E - ONECUT3 Antibody AO1836a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

    detail
  • WB - ONECUT3 Antibody AO1836a
    Figure 1: Western blot analysis using ONECUT3 mAb against human ONECUT3 recombinant protein. (Expected MW is 38.2 kDa)
    detail
  • WB - ONECUT3 Antibody AO1836a
    Figure 2: Western blot analysis using ONECUT3 mAb against HEK293 (1) and ONECUT3 (AA: 350-455)-hIgGFc transfected HEK293 (2) cell lysate.
    detail
  • IHC - ONECUT3 Antibody AO1836a
    Figure 3: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using ONECUT3 mouse mAb with DAB staining.
    detail
  • IHC - ONECUT3 Antibody AO1836a
    Figure 4: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using ONECUT3 mouse mAb with DAB staining.
    detail
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, E
Primary Accession O60422
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 10B2F5
Isotype IgG1
Calculated MW 50kDa
Description The protein encoded by this gene is a transcriptional activator. It can bind the consensus DNA sequence 5'-DHWATTGAYTWWD-3' on a variety of gene promoters such as those of HNF3B and TTR(By similarity).
Immunogen Purified recombinant fragment of human ONECUT3 (AA: 350-455) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 390874
Other Names One cut domain family member 3, One cut homeobox 3, Transcription factor ONECUT-3, OC-3, ONECUT3
Dilution E~~1/10000
WB~~1/500 - 1/2000
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsONECUT3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ONECUT3
Function Transcriptional activator. Binds the consensus DNA sequence 5'-DHWATTGAYTWWD-3' on a variety of gene promoters such as those of HNF3B and TTR (By similarity).
Cellular Location Nucleus {ECO:0000255|PROSITE-ProRule:PRU00108, ECO:0000255|PROSITE-ProRule:PRU00374}
Research Areas
Citations (0)
citation

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Background

The product of this gene transfers fucose to N-acetyllactosamine polysaccharides to generate fucosylated carbohydrate structures. It catalyzes the synthesis of the non-sialylated antigen, Lewis x (CD15). ;

References

1. Biochem Biophys Res Commun. 2002 Apr 12;292(4):848-54.

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$ 385.00
Cat# AO1836a
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