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ACP5 Antibody

Purified Mouse Monoclonal Antibody

     
  • E - ACP5 Antibody AO1841a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

    detail
  • WB - ACP5 Antibody AO1841a
    Figure 1: Western blot analysis using ACP5 mAb against human ACP5 recombinant protein. (Expected MW is 37.3 kDa)
    detail
  • WB - ACP5 Antibody AO1841a
    Figure 2: Western blot analysis using ACP5 mAb against HEK293 (1) and ACP5 (AA: 221-325)-hIgGFc transfected HEK293 (2) cell lysate.
    detail
  • WB - ACP5 Antibody AO1841a
    Figure 3: Western blot analysis using ACP5 mouse mAb against A431 (1), T47D (2), HepG2 (3), MOLT4 (4), Jurkat (5) and Hela (6) cell lysate.
    detail
  • IHC - ACP5 Antibody AO1841a
    Figure 4: Immunohistochemical analysis of paraffin-embedded liver cancer tissues using ACP5 mouse mAb with DAB staining.
    detail
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, E
Primary Accession P13686
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 7E6A11
Isotype IgG1
Calculated MW 36.6kDa
Description This gene encodes an iron containing glycoprotein which catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is the most basic of the acid phosphatases and is the only form not inhibited by L(+)-tartrate.
Immunogen Purified recombinant fragment of human ACP5 (AA: 221-325) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 54
Other Names Tartrate-resistant acid phosphatase type 5, TR-AP, 3.1.3.2, Tartrate-resistant acid ATPase, TrATPase, Type 5 acid phosphatase, ACP5
Dilution E~~1/10000
WB~~1/500 - 1/2000
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsACP5 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ACP5
Function Involved in osteopontin/bone sialoprotein dephosphorylation. Its expression seems to increase in certain pathological states such as Gaucher and Hodgkin diseases, the hairy cell, the B-cell, and the T- cell leukemias.
Cellular Location Lysosome.
Research Areas
Citations (0)
citation

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Background

The protein encoded by this gene is a small cellular or pericellular matrix proteoglycan that is closely related in structure to biglycan protein. The encoded protein and biglycan are thought to be the result of a gene duplication. This protein is a component of connective tissue, binds to type I collagen fibrils, and plays a role in matrix assembly. It contains one attached glycosaminoglycan chain. This protein is capable of suppressing the growth of various tumor cell lines. There are multiple alternatively spliced transcript variants known for this gene. This gene is a candidate gene for Marfan syndrome. ;

References

1. Clin Chim Acta. 2011 May 12;412(11-12):963-9. 2. Eur J Gynaecol Oncol. 2011;32(6):615-8.

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$ 385.00
Cat# AO1841a
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