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ZEB1 Antibody

Purified Mouse Monoclonal Antibody

  • E - ZEB1 Antibody AO1850a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - ZEB1 Antibody AO1850a
    Figure 1: Western blot analysis using ZEB1 mAb against human ZEB1 recombinant protein. (Expected MW is 41.7 kDa)
  • WB - ZEB1 Antibody AO1850a
    Figure 2: Western blot analysis using ZEB1 mAb against HEK293 (1) and ZEB1 (AA: 967-1108)-hIgGFc transfected HEK293 (2) cell lysate.
  • IF - ZEB1 Antibody AO1850a
    Figure 3: Immunofluorescence analysis of Hela cells using ZEB1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • FC - ZEB1 Antibody AO1850a
    Figure 4: Flow cytometric analysis of Hela cells using ZEB1 mouse mAb (green) and negative control (red).
  • IHC - ZEB1 Antibody AO1850a
    Figure 5: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using ZEB1 mouse mAb with DAB staining.
  • IHC - ZEB1 Antibody AO1850a
    Figure 6: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using ZEB1 mouse mAb with DAB staining.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P37275
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 2A8H3
Isotype IgG1
Calculated MW 124kDa
Description This gene encodes a zinc finger transcription factor. The encoded protein likely plays a role in transcriptional repression of interleukin 2. Mutations in this gene have been associated with posterior polymorphous corneal dystrophy-3 and late-onset Fuchs endothelial corneal dystrophy. Alternatively spliced transcript variants encoding different isoforms have been described.
Immunogen Purified recombinant fragment of human ZEB1 (AA: 967-1108) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 6935
Other Names Zinc finger E-box-binding homeobox 1, NIL-2-A zinc finger protein, Negative regulator of IL2, Transcription factor 8, TCF-8, ZEB1, AREB6, TCF8
Dilution E~~1/10000
WB~~1/500 - 1/2000
IF~~1/200 - 1/1000
FC~~1/200 - 1/400
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsZEB1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ZEB1
Synonyms AREB6, TCF8
Function Acts as a transcriptional repressor. Inhibits interleukin-2 (IL-2) gene expression. Enhances or represses the promoter activity of the ATP1A1 gene depending on the quantity of cDNA and on the cell type. Represses E-cadherin promoter and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. Represses BCL6 transcription in the presence of the corepressor CTBP1. Positively regulates neuronal differentiation. Represses RCOR1 transcription activation during neurogenesis. Represses transcription by binding to the E box (5'-CANNTG-3'). Promotes tumorigenicity by repressing stemness-inhibiting microRNAs.
Cellular Location Nucleus
Tissue Location Colocalizes with SMARCA4/BRG1 in E-cadherin- negative cells from established lines, and stroma of normal colon as well as in de-differentiated epithelial cells at the invasion front of colorectal carcinomas (at protein level). Expressed in heart and skeletal muscle, but not in liver, spleen, or pancreas
Research Areas
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CD22 may be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules ;


1. J Cancer Res Clin Oncol. 2012 Aug;138(8):1329-38. 2. Mol Cell Biochem. 2012 Jul;366(1-2):223-9.

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$ 385.00
Cat# AO1850a
(40 western blots)
Availability: 7-10 days
Bulk Size
Seasonal Special on Bulk Order
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