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SYCP3 Antibody

Purified Mouse Monoclonal Antibody

     
  • E - SYCP3 Antibody AO1855a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - SYCP3 Antibody AO1855a
    Figure 1: Western blot analysis using SYCP3 mAb against human SYCP3 recombinant protein. (Expected MW is 37.2 kDa)
  • WB - SYCP3 Antibody AO1855a
    Figure 2: Western blot analysis using SYCP3 mAb against HEK293 (1) and SYCP3 (AA: 27-128)-hIgGFc transfected HEK293 (2) cell lysate.
  • IF - SYCP3 Antibody AO1855a
    Figure 3: Immunofluorescence analysis of HepG2 cells using SYCP3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • FC - SYCP3 Antibody AO1855a
    Figure 4: Flow cytometric analysis of Jurkat cells using SYCP3 mouse mAb (green) and negative control (red).
  • IHC - SYCP3 Antibody AO1855a
    Figure 5: Immunohistochemical analysis of paraffin-embedded endometrial cancer tissues using SYCP3 mouse mAb with DAB staining.
  • IHC - SYCP3 Antibody AO1855a
    Figure 6: Immunohistochemical analysis of paraffin-embedded kidney tissues using SYCP3 mouse mAb with DAB staining.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, FC, ICC, E
Primary Accession Q8IZU3
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 6F9B6
Isotype IgG1
Calculated MW 27.7kDa
Description This gene encodes an essential structural component of the synaptonemal complex. This complex is involved in synapsis, recombination and segregation of meiotic chromosomes. Mutations in this gene are associated with azoospermia in males and susceptibility to pregnancy loss in females. Alternate splicing results in multiple transcript variants that encode the same protein.
Immunogen Purified recombinant fragment of human SYCP3 (AA: 27-128) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 50511
Other Names Synaptonemal complex protein 3, SCP-3, SYCP3, SCP3
Dilution E~~1/10000
WB~~1/500 - 1/2000
IF~~1/200 - 1/1000
FC~~1/200 - 1/400
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSYCP3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SYCP3
Synonyms SCP3
Function Component of the synaptonemal complexes (SCS), formed between homologous chromosomes during meiotic prophase. Required for centromere pairing during meiosis in male germ cells (By similarity). Required for normal meiosis during spermatogenesis and male fertility (PubMed:14643120). Plays a lesser role in female fertility. Required for efficient phosphorylation of HORMAD1 and HORMAD2 (By similarity).
Cellular Location Nucleus {ECO:0000250|UniProtKB:Q60547}. Chromosome {ECO:0000250|UniProtKB:Q60547}. Chromosome, centromere {ECO:0000250|UniProtKB:Q60547}. Note=It is present in early unpaired cores, in the lateral domains of the synaptonemal complex and in the chromosome cores when they separate at diplotene. It is found axial to the metaphase I chromosomes and in association with pairs of sister centromeres. The centromere-associated protein becomes dissociated from the centromeres at anaphase II and is not found in mitotic metaphase centromeres {ECO:0000250|UniProtKB:Q60547}
Tissue Location Testis-specific.
Research Areas
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Background

This gene encodes an essential structural component of the synaptonemal complex. This complex is involved in synapsis, recombination and segregation of meiotic chromosomes. Mutations in this gene are associated with azoospermia in males and susceptibility to pregnancy loss in females. Alternate splicing results in multiple transcript variants that encode the same protein.

References

1. Hum Pathol. 2013 Apr;44(4):472-9. 2. Cytogenet Genome Res. 2010;128(1-3):162-8.

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$ 385.00
Cat# AO1855a
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