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PAPLN Antibody

Purified Mouse Monoclonal Antibody

     
  • E - PAPLN Antibody AO1859a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - PAPLN Antibody AO1859a
    Figure 1: Western blot analysis using PAPLN mAb against human PAPLN recombinant protein. (Expected MW is 36.4 kDa)
  • WB - PAPLN Antibody AO1859a
    Figure 2: Western blot analysis using PAPLN mAb against HEK293 (1) and PAPLN (AA: 766-870)-hIgGFc transfected HEK293 (2) cell lysate.
  • SPECIFICATION
  • CITATIONS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession O95428
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 5F2D10
Isotype IgG1
Calculated MW 137.7kDa
Description Papilin is an extracellular matrix glycoprotein involved in, thin matrix layers during gastrulation, matrix associated with wandering, phagocytic hemocytes, basement membranes and space-filling matrix during Drosophila development.Determination of its cDNA sequence led to the identification of Caenorhabditis and mammalian papilins. A distinctly conserved 'papilin cassette' of domains at the amino-end of papilins is also the carboxyl-end of the ADAMTS subgroup of secreted, matrix-associated metalloproteinases; this cassette contains one thrombospondin type 1 (TSR) domain, a specific cysteine-rich domain and several partial TSR domains. In vitro, papilin non-competitively inhibits procollagen N-proteinase, an ADAMTS metalloproteinase.
Immunogen Purified recombinant fragment of human PAPLN (AA: 766-870) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 89932
Other Names Papilin, PAPLN
Dilution E~~1/10000
WB~~1/500 - 1/2000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPAPLN Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PAPLN
Cellular Location Secreted.
Research Areas
Citations (0)

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Background

Papilin is an extracellular matrix glycoprotein involved in, thin matrix layers during gastrulation, matrix associated with wandering, phagocytic hemocytes, basement membranes and space-filling matrix during Drosophila development.Determination of its cDNA sequence led to the identification of Caenorhabditis and mammalian papilins. A distinctly conserved 'papilin cassette' of domains at the amino-end of papilins is also the carboxyl-end of the ADAMTS subgroup of secreted, matrix-associated metalloproteinases; this cassette contains one thrombospondin type 1 (TSR) domain, a specific cysteine-rich domain and several partial TSR domains. In vitro, papilin non-competitively inhibits procollagen N-proteinase, an ADAMTS metalloproteinase. ; ;

References

1. Int J Biochem Cell Biol. 2004 Jun; 36(6):1079-84. 2. Development. 2000 Dec;127(24):5475-85.

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$ 385.00
Cat# AO1859a
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