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PTPRC Antibody

Purified Mouse Monoclonal Antibody

  • E - PTPRC Antibody AO1889a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - PTPRC Antibody AO1889a
    Figure 1: Western blot analysis using PTPRC mAb against human PTPRC (AA: 928-989) recombinant protein. (Expected MW is 33 kDa)
  • WB - PTPRC Antibody AO1889a
    Figure 2: Western blot analysis using PTPRC mAb against HEK293 (1) and PTPRC (AA: 928-989)-hIgGFc transfected HEK293 (2) cell lysate.
  • IHC - PTPRC Antibody AO1889a
    Figure 3: Immunohistochemical analysis of paraffin-embedded breast cancer tissues using PTPRC mouse mAb with DAB staining.
  • IHC - PTPRC Antibody AO1889a
    Figure 4: Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using PTPRC mouse mAb with DAB staining.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P08575
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 5D3A3
Isotype IgG1
Calculated MW 147.3kDa
Description The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported.
Immunogen Purified recombinant fragment of human PTPRC (AA: 928-989) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 5788
Other Names Receptor-type tyrosine-protein phosphatase C,, Leukocyte common antigen, L-CA, T200, CD45, PTPRC, CD45
Dilution E~~1/10000
WB~~1/500 - 1/2000
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPTPRC Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms CD45
Function Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity (By similarity).
Cellular Location Membrane; Single-pass type I membrane protein Membrane raft. Note=Colocalized with DPP4 in membrane rafts
Research Areas
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The protein encoded by this gene is a surface antigen that is preferentially expressed on monocytes/macrophages. It cooperates with other proteins to mediate the innate immune response to bacterial lipopolysaccharide. Alternative splicing results in multiple transcript variants encoding the same protein. ; ;


1. Biochem Biophys Res Commun. 2012 Mar 23;419(4):708-14. 2. Blood. 2009 Jun 4;113(23):5905-10.

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$ 385.00
Cat# AO1889a
(40 western blots)
Availability: 7-10 days
Bulk Size
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