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B2M Antibody

Purified Mouse Monoclonal Antibody

  • E - B2M Antibody AO1895a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - B2M Antibody AO1895a
    Figure 1: Western blot analysis using B2M mAb against human B2M (AA: 21-100) recombinant protein. (Expected MW is 35.4 kDa)
  • WB - B2M Antibody AO1895a
    Figure 2: Western blot analysis using B2M mAb against HEK293 (1) and B2M (AA: 21-100)-hIgGFc transfected HEK293 (2) cell lysate.
  • WB - B2M Antibody AO1895a
    Figure 3: Western blot analysis using B2M mouse mAb against Hela (1), HEK293 (2), HepG2 (3),RAJI (4), A431 (5) and Jurkat (6) cell lysate.
  • FC - B2M Antibody AO1895a
    Figure 4: Flow cytometric analysis of A431 cells using B2M mouse mAb (green) and negative control (red).
  • IHC - B2M Antibody AO1895a
    Figure 5: Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using B2M mouse mAb with DAB staining.
  • IHC - B2M Antibody AO1895a
    Figure 6: Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using B2M mouse mAb with DAB staining.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P61769
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 3G5H8
Isotype IgG2a
Calculated MW 13.7kDa
Description This gene encodes a serum protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells. The protein has a predominantly beta-pleated sheet structure that can form amyloid fibrils in some pathological conditions. A mutation in this gene has been shown to result in hypercatabolic hypoproteinemia.
Immunogen Purified recombinant fragment of human B2M (AA: 21-100) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide.
Additional Information
Gene ID 567
Other Names Beta-2-microglobulin, Beta-2-microglobulin form pI 5.3, B2M
Dilution E~~1/10000
WB~~1/500 - 1/2000
FC~~1/200 - 1/400
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsB2M Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name B2M
Function Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system.
Cellular Location Secreted. Note=Detected in serum and urine
Research Areas
Citations (0)

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The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit is one of the gamma regulatory subunits of AMPK. Alternatively spliced transcript variants encoding distinct isoforms have been observed. ;


1. Cancer Immunol Immunother. 2012 Sep;61(9):1359-71. 2. Lupus. 2012 Sep;21(10):1098-104.

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$ 385.00
Cat# AO1895a
(40 western blots)
Availability: 7-10 days
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