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NFE2L2 Antibody

Purified Mouse Monoclonal Antibody

  • E - NFE2L2 Antibody AO1896a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - NFE2L2 Antibody AO1896a
    Figure 1: Western blot analysis using NFE2L2 mAb against human NFE2L2 (AA: 356-589) recombinant protein. (Expected MW is 52.1 kDa)
  • WB - NFE2L2 Antibody AO1896a
    Figure 2: Western blot analysis using NFE2L2 mAb against HEK293 (1) and NFE2L2 (AA: 356-589)-hIgGFc transfected HEK293 (2) cell lysate.
  • IHC - NFE2L2 Antibody AO1896a
    Figure 3: Immunohistochemical analysis of paraffin-embedded stomach cancer tissues using NFE2L2 mouse mAb with DAB staining.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q16236
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 1A6G6
Isotype IgG1
Calculated MW 67.8kDa
Description This gene encodes a transcription factor which is a member of a small family of basic leucine zipper (bZIP) proteins. The encoded transcription factor regulates genes which contain antioxidant response elements (ARE) in their promoters; many of these genes encode proteins involved in response to injury and inflammation which includes the production of free radicals. Multiple transcript variants encoding different isoforms have been found for this gene.
Immunogen Purified recombinant fragment of human NFE2L2 (AA: 356-589) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide.
Additional Information
Gene ID 4780
Other Names Nuclear factor erythroid 2-related factor 2, NF-E2-related factor 2, NFE2-related factor 2, HEBP1, Nuclear factor, erythroid derived 2, like 2, NFE2L2, NRF2
Dilution E~~1/10000
WB~~1/500 - 1/2000
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNFE2L2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NFE2L2
Synonyms NRF2
Function Transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. Important for the coordinated up-regulation of genes in response to oxidative stress. May be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region.
Cellular Location Cytoplasm, cytosol. Nucleus. Note=Cytosolic under unstressed conditions, translocates into the nucleus upon induction by electrophilic agents
Tissue Location Widely expressed. Highest expression in adult muscle, kidney, lung, liver and in fetal muscle
Research Areas
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EIF2A is a 65-kD protein that catalyzes the formation of puromycin-sensitive 80S preinitiation complexes.


1. J Exp Clin Cancer Res. 2012 Aug 19;31:66. 2. Physiol Genomics. 2012 Aug 1;44(15):754-63.

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$ 385.00
Cat# AO1896a
(40 western blots)
Availability: 7-10 days
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