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B2M Antibody

Purified Mouse Monoclonal Antibody

  • E - B2M Antibody AO1899a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - B2M Antibody AO1899a
    Figure 1: Western blot analysis using B2M mAb against human B2M (AA: 21-100) recombinant protein. (Expected MW is 35.4 kDa)
  • WB - B2M Antibody AO1899a
    Figure 2: Western blot analysis using B2M mAb against HEK293 (1) and B2M (AA: 21-100)-hIgGFc transfected HEK293 (2) cell lysate.
  • IF - B2M Antibody AO1899a
    Figure 3: Immunofluorescence analysis of Hela cells using B2M mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Secondary antibody from Fisher (Cat#: 35503)
  • FC - B2M Antibody AO1899a
    Figure 4: Flow cytometric analysis of A431 cells using B2M mouse mAb (green) and negative control (red).
  • IHC - B2M Antibody AO1899a
    Figure 5: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using B2M mouse mAb with DAB staining.
  • IHC - B2M Antibody AO1899a
    Figure 6: Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using B2M mouse mAb with DAB staining.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P61769
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 4G5A1
Isotype IgG1
Calculated MW 13.7kDa
Description This gene encodes a serum protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells. The protein has a predominantly beta-pleated sheet structure that can form amyloid fibrils in some pathological conditions. A mutation in this gene has been shown to result in hypercatabolic hypoproteinemia.
Immunogen Purified recombinant fragment of human B2M (AA: 21-100) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide.
Additional Information
Gene ID 567
Other Names Beta-2-microglobulin, Beta-2-microglobulin form pI 5.3, B2M
Dilution E~~1/10000
WB~~1/500 - 1/2000
IF~~1/200 - 1/1000
FC~~1/200 - 1/400
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsB2M Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name B2M
Function Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system.
Cellular Location Secreted. Note=Detected in serum and urine
Research Areas
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The protein encoded by this gene is a transmembrane (type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. The syndecans mediate cell binding, cell signaling, and cytoskeletal organization and syndecan receptors are required for internalization of the HIV-1 tat protein. The syndecan-1 protein functions as an integral membrane protein and participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. Altered syndecan-1 expression has been detected in several different tumor types. While several transcript variants may exist for this gene, the full-length natures of only two have been described to date. These two represent the major variants of this gene and encode the same protein. ; ; ; ; ;


1. Cancer Immunol Immunother. 2012 Sep;61(9):1359-71. 2. Lupus. 2012 Sep;21(10):1098-104.

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$ 385.00
Cat# AO1899a
(40 western blots)
Availability: 7-10 days
Bulk Size
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