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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   PRKAG1 Antibody   

PRKAG1 Antibody

Purified Mouse Monoclonal Antibody

  • E - PRKAG1 Antibody AO1900a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - PRKAG1 Antibody AO1900a
    Figure 1: Western blot analysis using PRKAG1 mAb against human PRKAG1 (AA: 230-331) recombinant protein. (Expected MW is 37.4 kDa)
  • WB - PRKAG1 Antibody AO1900a
    Figure 2: Western blot analysis using PRKAG1 mAb against HEK293 (1) and PRKAG1 (AA: 230-331)-hIgGFc transfected HEK293 (2) cell lysate.
  • IHC - PRKAG1 Antibody AO1900a
    Figure 3: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using PRKAG1 mouse mAb with DAB staining.
  • IHC - PRKAG1 Antibody AO1900a
    Figure 4: Immunohistochemical analysis of paraffin-embedded breast cancer tissues using PRKAG1 mouse mAb with DAB staining.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P54619
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 4A1G9
Isotype IgG1
Calculated MW 37.6kDa
Description The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit is one of the gamma regulatory subunits of AMPK. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
Immunogen Purified recombinant fragment of human PRKAG1 (AA: 230-331) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide.
Additional Information
Gene ID 5571
Other Names 5'-AMP-activated protein kinase subunit gamma-1, AMPK gamma1, AMPK subunit gamma-1, AMPKg, PRKAG1
Dilution E~~1/10000
WB~~1/500 - 1/2000
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPRKAG1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Function AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Gamma non-catalytic subunit mediates binding to AMP, ADP and ATP, leading to activate or inhibit AMPK: AMP-binding results in allosteric activation of alpha catalytic subunit (PRKAA1 or PRKAA2) both by inducing phosphorylation and preventing dephosphorylation of catalytic subunits. ADP also stimulates phosphorylation, without stimulating already phosphorylated catalytic subunit. ATP promotes dephosphorylation of catalytic subunit, rendering the AMPK enzyme inactive.
Research Areas
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The multi-pass membrane protein encoded by this gene belongs to the G-protein coupled receptor 3 family and GABA-B receptor subfamily. The GABA-B receptors inhibit neuronal activity through G protein-coupled second-messenger systems, which regulate the release of neurotransmitters, and the activity of ion channels and adenylyl cyclase. This receptor subunit forms an active heterodimeric complex with GABA-B receptor subunit 1, neither of which is effective on its own. Allelic variants of this gene have been associated with nicotine dependence. ; ; ; ;


1. Circ Res. 2012 Aug 31;111(6):800-14. 2. Circ Res. 2012 Apr 27;110(9):1192-201.

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$ 385.00
Cat# AO1900a
(40 western blots)
Availability: 7-10 days
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