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BDNF Antibody

Purified Mouse Monoclonal Antibody

  • E - BDNF Antibody AO1909a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - BDNF Antibody AO1909a
    Figure 1: Western blot analysis using BDNF mAb against human BDNF (AA: 19-248) recombinant protein. (Expected MW is 51.7 kDa)
  • WB - BDNF Antibody AO1909a
    Figure 2: Western blot analysis using BDNF mAb against HEK293 (1) and BDNF (AA: 19-248)-hIgGFc transfected HEK293 (2) cell lysate.
  • WB - BDNF Antibody AO1909a
    Figure 3: Western blot analysis using BDNF mouse mAb against SK-N-SH (1) cell lysate.
  • IF - BDNF Antibody AO1909a
    Figure 4: Immunofluorescence analysis of Hela cells using BDNF mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Secondary antibody from Fisher (Cat#: 35503)
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P23560
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 3D9C5
Isotype IgG1
Calculated MW 27.8kDa
Description The protein encoded by this gene is a member of the nerve growth factor family. It is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression of this gene is reduced in both Alzheimer's and Huntington disease patients. This gene may play a role in the regulation of stress response and in the biology of mood disorders. Multiple transcript variants encoding distinct isoforms have been described for this gene.
Immunogen Purified recombinant fragment of human BDNF (AA: 19-248) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide.
Additional Information
Gene ID 627
Other Names Brain-derived neurotrophic factor, BDNF, Abrineurin, BDNF
Dilution E~~1/10000
WB~~1/500 - 1/2000
IF~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsBDNF Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Function During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.
Cellular Location Secreted.
Tissue Location Brain. Highly expressed in hippocampus, amygdala, cerebral cortex and cerebellum. Also expressed in heart, lung, skeletal muscle, testis, prostate and placenta
Research Areas
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1. Br J Cancer. 2013 Jan 15;108(1):121-30. 2. PLoS One. 2012;7(8):e42676.

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$ 385.00
Cat# AO1909a
(40 western blots)
Availability: 7-10 days
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