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NT5E Antibody

Purified Mouse Monoclonal Antibody

  • E - NT5E Antibody AO1910a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - NT5E Antibody AO1910a
    Figure 1: Western blot analysis using NT5E mAb against human NT5E (AA: ) recombinant protein. (Expected MW is 26.6 kDa)
  • WB - NT5E Antibody AO1910a
    Figure 2: Western blot analysis using NT5E mAb against HEK293 (1) and NT5E (AA: 30-250)-hIgGFc transfected HEK293 (2) cell lysate.
  • WB - NT5E Antibody AO1910a
    Figure 3: Western blot analysis using NT5E mouse mAb against A431 (1) cell lysate.
  • IHC - NT5E Antibody AO1910a
    Figure 4: Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using NT5E mouse mAb with DAB staining.
  • IHC - NT5E Antibody AO1910a
    Figure 5: Immunohistochemical analysis of paraffin-embedded esophagus cancer tissues using NT5E mouse mAb with DAB staining.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P21589
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 4G6B10
Isotype IgG1
Calculated MW 63.4kDa
Description The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.
Immunogen Purified recombinant fragment of human NT5E (AA: 30-250) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide.
Additional Information
Gene ID 4907
Other Names 5'-nucleotidase, 5'-NT,, Ecto-5'-nucleotidase, CD73, NT5E, NT5, NTE
Dilution E~~1/10000
WB~~1/500 - 1/2000
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNT5E Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NT5E
Synonyms NT5, NTE
Function Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Exhibits AMP-, NAD-, and NMN-nucleosidase activities.
Cellular Location Cell membrane; Lipid-anchor, GPI-anchor.
Research Areas
Citations (0)

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This gene is a member of the solute carrier family 2 (facilitated glucose transporter) family and encodes a protein that functions as an insulin-regulated facilitative glucose transporter. In the absence of insulin, this integral membrane protein is sequestered within the cells of muscle and adipose tissue. Within minutes of insulin stimulation, the protein moves to the cell surface and begins to transport glucose across the cell membrane. Mutations in this gene have been associated with noninsulin-dependent diabetes mellitus (NIDDM). ; ;


1. Appl Immunohistochem Mol Morphol. 2012 Mar;20(2):103-7. 2. J Surg Oncol. 2012 Aug 1;106(2):130-7.

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$ 385.00
Cat# AO1910a
(40 western blots)
Availability: 7-10 days
Bulk Size
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