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PLA2G12A Antibody

Purified Mouse Monoclonal Antibody

  • E - PLA2G12A Antibody AO1927a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - PLA2G12A Antibody AO1927a
    Figure 1: Western blot analysis using PLA2G12A mAb against human PLA2G12A (AA: 21-189) recombinant protein. (Expected MW is 44.5 kDa)
  • WB - PLA2G12A Antibody AO1927a
    Figure 2: Western blot analysis using PLA2G12A mAb against HEK293 (1) and PLA2G12A (AA: 21-189)-hIgGFc transfected HEK293 (2) cell lysate.
  • FC - PLA2G12A Antibody AO1927a
    Figure 3: Flow cytometric analysis of Hela cells using PLA2G12A mouse mAb (green) and negative control (red).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9BZM1
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 7C7C9
Isotype IgG1
Calculated MW 21kDa
Description Secreted phospholipase A2 (sPLA2) enzymes liberate arachidonic acid from phospholipids for production of eicosanoids and exert a variety of physiologic and pathologic effects. Group XII sPLA2s, such as PLA2G12A, have relatively low specific activity and are structurally and functionally distinct from other sPLA2s
Immunogen Purified recombinant fragment of human PLA2G12A (AA: 21-189) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide.
Additional Information
Gene ID 81579
Other Names Group XIIA secretory phospholipase A2, GXII sPLA2, sPLA2-XII,, Phosphatidylcholine 2-acylhydrolase 12A, PLA2G12A, PLA2G12
Dilution E~~1/10000
WB~~1/500 - 1/2000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPLA2G12A Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PLA2G12A
Synonyms PLA2G12
Function PA2 catalyzes the calcium-dependent hydrolysis of the 2- acyl groups in 3-sn-phosphoglycerides. Does not exhibit detectable activity toward sn-2-arachidonoyl- or linoleoyl- phosphatidylcholine or -phosphatidylethanolamine.
Cellular Location Secreted. Cytoplasm
Tissue Location Abundantly expressed in heart, skeletal muscle, kidney, liver and pancreas
Research Areas
Citations (0)

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The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. ; ;


1. J Biol Chem. 2003 Mar 21;278(12):10657-67. 2. Biochemistry. 2003 Oct 7;42(39):11494-503.

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$ 385.00
Cat# AO1927a
(40 western blots)
Availability: 7-10 days
Bulk Size
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