|Application ||WB, IHC, FC, E|
|Description||Secreted phospholipase A2 (sPLA2) enzymes liberate arachidonic acid from phospholipids for production of eicosanoids and exert a variety of physiologic and pathologic effects. Group XII sPLA2s, such as PLA2G12A, have relatively low specific activity and are structurally and functionally distinct from other sPLA2s|
|Immunogen||Purified recombinant fragment of human PLA2G12A (AA: 21-189) expressed in E. Coli.|
|Formulation||Purified antibody in PBS with 0.05% sodium azide.|
|Other Names||Group XIIA secretory phospholipase A2, GXII sPLA2, sPLA2-XII, 18.104.22.168, Phosphatidylcholine 2-acylhydrolase 12A, PLA2G12A, PLA2G12|
WB~~1/500 - 1/2000
FC~~1/200 - 1/400
IHC~~1/200 - 1/1000
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||PLA2G12A Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||PA2 catalyzes the calcium-dependent hydrolysis of the 2- acyl groups in 3-sn-phosphoglycerides. Does not exhibit detectable activity toward sn-2-arachidonoyl- or linoleoyl- phosphatidylcholine or -phosphatidylethanolamine.|
|Cellular Location||Secreted. Cytoplasm|
|Tissue Location||Abundantly expressed in heart, skeletal muscle, kidney, liver and pancreas|
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The protein encoded by this gene is an isozyme of very long-chain acyl-CoA synthetase (VLCS). It is capable of activating very long-chain fatty-acids containing 24- and 26-carbons. It is expressed in liver and associated with endoplasmic reticulum but not with peroxisomes. Its primary role is in fatty acid elongation or complex lipid synthesis rather than in degradation. This gene has a mouse ortholog. ; ; ;
1. J Biol Chem. 2003 Mar 21;278(12):10657-67. 2. Biochemistry. 2003 Oct 7;42(39):11494-503.
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