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TUBE1 Antibody

Purified Mouse Monoclonal Antibody

     
  • E - TUBE1 Antibody AO1948a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

    detail
  • WB - TUBE1 Antibody AO1948a
    Figure 1: Western blot analysis using TUBE1 mAb against human TUBE1 (AA: 314-472) recombinant protein. (Expected MW is 44.3 kDa)
    detail
  • WB - TUBE1 Antibody AO1948a
    Figure 2: Western blot analysis using TUBE1 mAb against HEK293 (1) and TUBE1 (AA: 314-472)-hIgGFc transfected HEK293 (2) cell lysate.
    detail
  • FC - TUBE1 Antibody AO1948a
    Figure 3: Flow cytometric analysis of Hela cells using TUBE1 mouse mAb (green) and negative control (red).
    detail
  • SPECIFICATION
  • CITATIONS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, FC, E
Primary Accession Q9UJT0
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 7G3B2
Isotype IgG1
Calculated MW 53kDa
Description This gene encodes a member of the tubulin superfamily. This protein localizes to the centriolar sub-distal appendages that are associated with the older of the two centrioles after centrosome duplication. This protein plays a central role in organization of the microtubules during centriole duplication. A pseudogene of this gene is found on chromosome 5.
Immunogen Purified recombinant fragment of human TUBE1 (AA: 314-472) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide.
Additional Information
Gene ID 51175
Other Names Tubulin epsilon chain, Epsilon-tubulin, TUBE1, TUBE
Dilution E~~1/10000
WB~~1/500 - 1/2000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTUBE1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TUBE1
Synonyms TUBE
Cellular Location Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Associated with pericentriolar material.
Research Areas
Citations (0)
citation

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Background

The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase (PDE) family, and PDE1 subfamily. Members of the PDE1 family are calmodulin-dependent PDEs that are stimulated by a calcium-calmodulin complex. This PDE has dual-specificity for the second messengers, cAMP and cGMP, with a preference for cGMP as a substrate. cAMP and cGMP function as key regulators of many important physiological processes. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. ; ;

References

1. Nat Cell Biol. 2003 Jan;5(1):71-6.2. Nat Cell Biol. 2000 Jan;2(1):30-5.

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$ 385.00
Cat# AO1948a
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