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RHOF Antibody

Purified Mouse Monoclonal Antibody

  • E - RHOF Antibody AO1955a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - RHOF Antibody AO1955a
    Figure 1: Western blot analysis using RHOF mAb against human RHOF (AA: 1-84) recombinant protein. (Expected MW is 34.9 kDa)
  • WB - RHOF Antibody AO1955a
    Figure 2: Western blot analysis using RHOF mAb against HEK293 (1) and RHOF (AA: 1-84)-hIgGFc transfected HEK293 (2) cell lysate.
  • FC - RHOF Antibody AO1955a
    Figure 3: Flow cytometric analysis of Hela cells using RHOF mouse mAb (green) and negative control (red).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9HBH0
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 1D4B6
Isotype IgG2a
Calculated MW 23.6kDa
Description RHOF is a protein-coding gene. Diseases associated with RHOF include mycosis fungoides, and lung cancer, and among its related super-pathways are Signaling by Rho GTPases and Signaling by GPCR. GO annotations related to this gene include GTP binding and GTPase activity. An important paralog of this gene is RHOB.
Immunogen Purified recombinant fragment of human RHOF (AA: 1-84) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide.
Additional Information
Gene ID 54509
Other Names Rho-related GTP-binding protein RhoF, Rho family GTPase Rif, Rho in filopodia, RHOF, ARHF, RIF
Dilution E~~1/10000
WB~~1/500 - 1/2000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRHOF Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms ARHF, RIF
Function Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. Causes the formation of thin, actin-rich surface projections called filopodia. Functions cooperatively with CDC42 and Rac to generate additional structures, increasing the diversity of actin- based morphology.
Cellular Location Cell membrane; Lipid-anchor; Cytoplasmic side. Cytoplasm, cytoskeleton
Research Areas
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Soluble guanylate cyclases are heterodimeric proteins that catalyze the conversion of GTP to 3',5'-cyclic GMP and pyrophosphate. The protein encoded by this gene is an alpha subunit of this complex and it interacts with a beta subunit to form the guanylate cyclase enzyme, which is activated by nitric oxide. Several transcript variants encoding a few different isoforms have been found for this gene. ; ;


1. Curr Biol. 2000 Nov 2;10(21):1387-90.2. Biochem Soc Trans. 2012 Feb;40(1):268-72.

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$ 385.00
Cat# AO1955a
(40 western blots)
Availability: 7-10 days
Bulk Size
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