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RAP1GAP Antibody

Purified Mouse Monoclonal Antibody

  • E - RAP1GAP Antibody AO1974a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - RAP1GAP Antibody AO1974a
    Figure 1: Western blot analysis using RAP1GAP mAb against human RAP1GAP (AA: 412-678) recombinant protein. (Expected MW is 53.9 kDa)
  • WB - RAP1GAP Antibody AO1974a
    Figure 2: Western blot analysis using RAP1GAP mAb against HEK293 (1) and RAP1GAP (AA: 412-678)-hIgGFc transfected HEK293 (2) cell lysate.
  • FC - RAP1GAP Antibody AO1974a
    Figure 3: Flow cytometric analysis of A431 cells using RAP1GAP mouse mAb (green) and negative control (red).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P47736
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 2G7B6
Isotype IgG2b
Calculated MW 73.4kDa
Description This gene encodes a type of GTPase-activating-protein (GAP) that down-regulates the activity of the ras-related RAP1 protein. RAP1 acts as a molecular switch by cycling between an inactive GDP-bound form and an active GTP-bound form. The product of this gene, RAP1GAP, promotes the hydrolysis of bound GTP and hence returns RAP1 to the inactive state whereas other proteins, guanine nucleotide exchange factors (GEFs), act as RAP1 activators by facilitating the conversion of RAP1 from the GDP- to the GTP-bound form. In general, ras subfamily proteins, such as RAP1, play key roles in receptor-linked signaling pathways that control cell growth and differentiation. RAP1 plays a role in diverse processes such as cell proliferation, adhesion, differentiation, and embryogenesis. Alternative splicing results in multiple transcript variants encoding distinct proteins.
Immunogen Purified recombinant fragment of human RAP1GAP (AA: 412-678) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide.
Additional Information
Gene ID 5909
Other Names Rap1 GTPase-activating protein 1, Rap1GAP, Rap1GAP1, RAP1GAP, KIAA0474, RAP1GA1
Dilution E~~1/10000
WB~~1/500 - 1/2000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRAP1GAP Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms KIAA0474, RAP1GA1
Function GTPase activator for the nuclear Ras-related regulatory protein RAP-1A (KREV-1), converting it to the putatively inactive GDP-bound state.
Cellular Location Golgi apparatus membrane; Peripheral membrane protein
Tissue Location Significant expression seen in the brain, kidney and pancreas. Abundant in the cerebral cortex and expressed at much lower levels in the spinal cord. Not detected in the lymphoid tissues.
Research Areas
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1. Endocr Relat Cancer. 2012 Jul 22;19(4):575-88.2. Cancer Lett. 2012 Jul 1;320(1):65-71.

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$ 385.00
Cat# AO1974a
(40 western blots)
Availability: 7-10 days
Bulk Size
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