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CCNA2 Antibody

Purified Mouse Monoclonal Antibody

  • E - CCNA2 Antibody AO1984a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - CCNA2 Antibody AO1984a
    Figure 1: Western blot analysis using CCNA2 mAb against human CCNA2 (AA: 105-233) recombinant protein. (Expected MW is 40.8 kDa)
  • WB - CCNA2 Antibody AO1984a
    Figure 2: Western blot analysis using CCNA2 mAb against HEK293 (1) and CCNA2 (AA: 105-233)-hIgGFc transfected HEK293 (2) cell lysate.
  • WB - CCNA2 Antibody AO1984a
    Figure 3: Western blot analysis using CCNA2 mouse mAb against Hela (1), HEK293 (2), Jurkat (3), K562 (4), SK-Br-3 (5), NIH/3T3 (6) cell lysate.
  • FC - CCNA2 Antibody AO1984a
    Figure 4: Flow cytometric analysis of A431 cells using CCNA2 mouse mAb (green) and negative control (red).
  • IHC - CCNA2 Antibody AO1984a
    Figure 5: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using CCNA2 mouse mAb with DAB staining.
  • IHC - CCNA2 Antibody AO1984a
    Figure 6: Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using CCNA2 mouse mAb with DAB staining.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P20248
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 6B4D11
Calculated MW 48.6kDa
Description The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. In contrast to cyclin A1, which is present only in germ cells, this cyclin is expressed in all tissues tested. This cyclin binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions.
Immunogen Purified recombinant fragment of human CCNA2 (AA: 105-233) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide.
Additional Information
Gene ID 890
Other Names Cyclin-A2, Cyclin-A, CCNA2, CCN1, CCNA
Dilution E~~1/10000
WB~~1/500 - 1/2000
FC~~1/200 - 1/400
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCCNA2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CCNA2
Synonyms CCN1, CCNA
Function Essential for the control of the cell cycle at the G1/S (start) and the G2/M (mitosis) transitions.
Cellular Location Nucleus. Cytoplasm. Note=Cytoplasmic when associated with SCAPER
Research Areas
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This gene is a member of the paired box (PAX) family of transcription factors. Members of the PAX family typically contain a paired box domain and a paired-type homeodomain. These genes play critical roles during fetal development. Mutations in paired box gene 3 are associated with Waardenburg syndrome, craniofacial-deafness-hand syndrome, and alveolar rhabdomyosarcoma. The translocation t(2;13)(q35;q14), which represents a fusion between PAX3 and the forkhead gene, is a frequent finding in alveolar rhabdomyosarcoma. Alternative splicing results in transcripts encoding isoforms with different C-termini. ; ;


1. Cancer. 2011 Sep 1;117(17):4080-91.2. J Phys Chem B. 2008 Jul 17;112(28):8346-53.

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$ 385.00
Cat# AO1984a
(40 western blots)
Availability: 7-10 days
Bulk Size
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