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WAS Antibody

Purified Mouse Monoclonal Antibody

  • E - WAS Antibody AO1986a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - WAS Antibody AO1986a
    Figure 1: Western blot analysis using WAS mAb against human WAS (AA: 57-170) recombinant protein. (Expected MW is 39 kDa)
  • WB - WAS Antibody AO1986a
    Figure 2: Western blot analysis using WAS mAb against HEK293 (1) and WAS (AA: 57-170)-hIgGFc transfected HEK293 (2) cell lysate.
  • FC - WAS Antibody AO1986a
    Figure 3: Flow cytometric analysis of Hela cells using WAS mouse mAb (green) and negative control (red).
  • IHC - WAS Antibody AO1986a
    Figure 4: Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using WAS mouse mAb with DAB staining.
  • IHC - WAS Antibody AO1986a
    Figure 5: Immunohistochemical analysis of paraffin-embedded colon cancer tissues using WAS mouse mAb with DAB staining.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P42768
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 7B10E4
Isotype IgG2a
Calculated MW 53kDa
Description The Wiskott-Aldrich syndrome (WAS) family of proteins share similar domain structure, and are involved in transduction of signals from receptors on the cell surface to the actin cytoskeleton. The presence of a number of different motifs suggests that they are regulated by a number of different stimuli, and interact with multiple proteins. Recent studies have demonstrated that these proteins, directly or indirectly, associate with the small GTPase, Cdc42, known to regulate formation of actin filaments, and the cytoskeletal organizing complex, Arp2/3. Wiskott-Aldrich syndrome is a rare, inherited, X-linked, recessive disease characterized by immune dysregulation and microthrombocytopenia, and is caused by mutations in the WAS gene. The WAS gene product is a cytoplasmic protein, expressed exclusively in hematopoietic cells, which show signalling and cytoskeletal abnormalities in WAS patients. A transcript variant arising as a result of alternative promoter usage, and containing a different 5' UTR sequence, has been described, however, its full-length nature is not known.
Immunogen Purified recombinant fragment of human WAS (AA: 57-170) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide.
Additional Information
Gene ID 7454
Other Names Wiskott-Aldrich syndrome protein, WASp, WAS, IMD2
Dilution E~~1/10000
WB~~1/500 - 1/2000
FC~~1/200 - 1/400
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsWAS Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name WAS
Synonyms IMD2
Function Effector protein for Rho-type GTPases. Regulates actin filament reorganization via its interaction with the Arp2/3 complex. Important for efficient actin polymerization. Possible regulator of lymphocyte and platelet function. Mediates actin filament reorganization and the formation of actin pedestals upon infection by pathogenic bacteria.
Cellular Location Cytoplasm, cytoskeleton.
Tissue Location Expressed predominantly in the thymus. Also found, to a much lesser extent, in the spleen
Research Areas
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Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a protein identified as belonging to both the 28S and the 39S subunits. Alternative splicing results in multiple transcript variants. Pseudogenes corresponding to this gene are found on chromosomes 4q, 6p, 6q, 7p, and 15q. ;


1. Mol Cell Biol. 2012 Aug;32(15):3153-63.2. Dis Markers. 2010;29(3-4):157-75.

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$ 385.00
Cat# AO1986a
(40 western blots)
Availability: 7-10 days
Bulk Size
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