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PKN1 Antibody

Purified Mouse Monoclonal Antibody

  • E - PKN1 Antibody AO1987a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - PKN1 Antibody AO1987a
    Figure 1: Western blot analysis using PKN1 mAb against human PKN1 (AA: 442-620) recombinant protein. (Expected MW is 45.7 kDa)
  • WB - PKN1 Antibody AO1987a
    Figure 2: Western blot analysis using PKN1 mAb against HEK293 (1) and PKN1 (AA: 442-620)-hIgGFc transfected HEK293 (2) cell lysate.
  • IF - PKN1 Antibody AO1987a

    Figure 3:Immunofluorescence analysis of Hela cells using PKN1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

  • FC - PKN1 Antibody AO1987a
    Figure 4: Flow cytometric analysis of Hela cells using PKN1 mouse mAb (green) and negative control (red).
  • IHC - PKN1 Antibody AO1987a
    Figure 5: Immunohistochemical analysis of paraffin-embedded colon cancer tissues using PKN1 mouse mAb with DAB staining.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q16512
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 4H10B1
Isotype IgG2b
Calculated MW 104kDa
Description The protein encoded by this gene belongs to the protein kinase C superfamily. This kinase is activated by Rho family of small G proteins and may mediate the Rho-dependent signaling pathway. This kinase can be activated by phospholipids and by limited proteolysis. The 3-phosphoinositide dependent protein kinase-1 (PDPK1/PDK1) is reported to phosphorylate this kinase, which may mediate insulin signals to the actin cytoskeleton. The proteolytic activation of this kinase by caspase-3 or related proteases during apoptosis suggests its role in signal transduction related to apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
Immunogen Purified recombinant fragment of human PKN1 (AA: 442-620) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide.
Additional Information
Gene ID 5585
Other Names Serine/threonine-protein kinase N1,, Protease-activated kinase 1, PAK-1, Protein kinase C-like 1, Protein kinase C-like PKN, Protein kinase PKN-alpha, Protein-kinase C-related kinase 1, Serine-threonine protein kinase N, PKN1, PAK1, PKN, PRK1, PRKCL1
Dilution E~~1/10000
WB~~1/500 - 1/2000
IF~~1/200 - 1/1000
FC~~1/200 - 1/400
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPKN1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PKN1
Synonyms PAK1, PKN, PRK1, PRKCL1
Function PKC-related serine/threonine-protein kinase involved in various processes such as regulation of the intermediate filaments of the actin cytoskeleton, cell migration, tumor cell invasion and transcription regulation. Regulates the cytoskeletal network by phosphorylating proteins such as VIM and neurofilament proteins NEFH, NEFL and NEFM, leading to inhibit their polymerization. Phosphorylates 'Ser-575', 'Ser-637' and 'Ser-669' of MAPT/Tau, lowering its ability to bind to microtubules, resulting in disruption of tubulin assembly. Acts as a key coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of 'Thr-11' of histone H3 (H3T11ph), a specific tag for epigenetic transcriptional activation that promotes demethylation of histone H3 'Lys-9' (H3K9me) by KDM4C/JMJD2C. Phosphorylates HDAC5, HDAC7 and HDAC9, leading to impair their import in the nucleus. Phosphorylates 'Thr-38' of PPP1R14A, 'Ser- 159', 'Ser-163' and 'Ser-170' of MARCKS, and GFAP. Able to phosphorylate RPS6 in vitro.
Cellular Location Cytoplasm. Nucleus. Endosome. Cell membrane; Peripheral membrane protein. Cleavage furrow. Midbody Note=Associates with chromatin in a ligand-dependent manner Localization to endosomes is mediated via its interaction with RHOB. Association to the cell membrane is dependent on Ser-374 phosphorylation. Accumulates during telophase at the cleavage furrow and finally concentrates around the midbody in cytokinesis
Tissue Location Found ubiquitously. Expressed in heart, brain, placenta, lung, skeletal muscle, kidney and pancreas. Expressed in numerous tumor cell lines, especially in breast tumor cells
Research Areas
Citations (0)

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T protein p53 binding protein 1 may have a role in checkpoint signaling during mitosis,enhance TP53-mediated transcriptional activation and play a role in the response to DNA damage. ;


1. J Biol Chem. 2013 Nov 29;288(48):34658-70.2. Hum Pathol. 2009 Oct;40(10):1434-40.

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$ 385.00
Cat# AO1987a
(40 western blots)
Availability: 7-10 days
Bulk Size
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