EPN1 Antibody
Purified Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, FC, E |
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Primary Accession | Q9Y6I3 |
Reactivity | Human |
Host | Mouse |
Clonality | Monoclonal |
Clone Names | 6F7F9 |
Isotype | IgG1 |
Calculated MW | 60.3kDa |
Description | The protein encoded by this gene binds clathrin and is involved in the endocytosis of clathrin-coated vesicles. Three transcript variants encoding different isoforms have been found for this gene. |
Immunogen | Purified recombinant fragment of human EPN1 (AA: 106-254) expressed in E. Coli. |
Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Gene ID | 29924 |
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Other Names | Epsin-1, EH domain-binding mitotic phosphoprotein, EPS-15-interacting protein 1, EPN1 |
Dilution | E~~1/10000 WB~~1/500 - 1/2000 FC~~1/200 - 1/400 |
Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | EPN1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | EPN1 |
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Function | Binds to membranes enriched in phosphatidylinositol 4,5- bisphosphate (PtdIns(4,5)P2). Modifies membrane curvature and facilitates the formation of clathrin-coated invaginations (By similarity). Regulates receptor-mediated endocytosis (PubMed:10557078, PubMed:10393179). |
Cellular Location | Cytoplasm. Cell membrane; Peripheral membrane protein. Nucleus. Membrane, clathrin-coated pit Note=Associated with the cytoplasmic membrane at sites where clathrin- coated pits are forming. Colocalizes with clathrin and AP-2 in a punctate pattern on the plasma membrane. Detected in presynaptic nerve terminals and in Golgi stacks. May shuttle to the nucleus when associated with ZBTB16/ZNF145 (By similarity). |
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Provided below are standard protocols that you may find useful for product applications.
References
1. J Biol Chem. 2011 Nov 25;286(47):40760-70. 2. Traffic. 2009 Feb;10(2):235-45.
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