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EPN1 Antibody

Purified Mouse Monoclonal Antibody

  • E - EPN1 Antibody AO2000a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - EPN1 Antibody AO2000a
    Figure 1: Western blot analysis using EPN1 mAb against human EPN1 (AA: 106-254) recombinant protein. (Expected MW is 42.4 kDa)
  • WB - EPN1 Antibody AO2000a
    Figure 2: Western blot analysis using EPN1 mAb against HEK293 (1) and EPN1 (AA: 106-254)-hIgGFc transfected HEK293 (2) cell lysate.
  • FC - EPN1 Antibody AO2000a
    Figure 3: Flow cytometric analysis of A431 cells using EPN1 mouse mAb (green) and negative control (red).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9Y6I3
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 6F7F9
Isotype IgG1
Calculated MW 60.3kDa
Description The protein encoded by this gene binds clathrin and is involved in the endocytosis of clathrin-coated vesicles. Three transcript variants encoding different isoforms have been found for this gene.
Immunogen Purified recombinant fragment of human EPN1 (AA: 106-254) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide.
Additional Information
Gene ID 29924
Other Names Epsin-1, EH domain-binding mitotic phosphoprotein, EPS-15-interacting protein 1, EPN1
Dilution E~~1/10000
WB~~1/500 - 1/2000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsEPN1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name EPN1
Function Binds to membranes enriched in phosphatidylinositol 4,5- bisphosphate (PtdIns(4,5)P2). Modifies membrane curvature and facilitates the formation of clathrin-coated invaginations (By similarity). Regulates receptor-mediated endocytosis.
Cellular Location Cytoplasm. Cell membrane; Peripheral membrane protein. Nucleus. Membrane, clathrin-coated pit Note=Associated with the cytoplasmic membrane at sites where clathrin-coated pits are forming. Colocalizes with clathrin and AP-2 in a punctate pattern on the plasma membrane. Detected in presynaptic nerve terminals and in Golgi stacks. May shuttle to the nucleus when associated with ZBTB16/ZNF145 (By similarity)
Research Areas
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1. J Biol Chem. 2011 Nov 25;286(47):40760-70. 2. Traffic. 2009 Feb;10(2):235-45.

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$ 385.00
Cat# AO2000a
(40 western blots)
Availability: 7-10 days
Bulk Size
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