|Application ||WB, FC, E|
|Description||Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.|
|Immunogen||Synthesized peptide of human HH3 (AA: ARTKQTAR(AcK)STG-C).|
|Formulation||Purified antibody in PBS with 0.05% sodium azide.|
|Other Names||Histone H3.1t, H3/t, H3t, H3/g, HIST3H3, H3FT|
WB~~1/500 - 1/2000
FC~~1/200 - 1/400
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||HH3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.|
|Cellular Location||Nucleus. Chromosome.|
|Tissue Location||Expressed in testicular cells.|
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Provided below are standard protocols that you may find useful for product applications.
1. J Cell Biochem. 2009 Oct 1;108(2):400-7.2. Trends Biochem Sci. 2005 Jul;30(7):357-9.
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