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PKHD1 Antibody

Purified Rat Monoclonal Antibody

  • E - PKHD1 Antibody AO2006a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - PKHD1 Antibody AO2006a
    Figure 1: Western blot analysis using PKHD1 mAb against mouse PKHD1(AA: 3878-4060) recombinant protein. (Expected MW is 23 kDa)
  • IF - PKHD1 Antibody AO2006a

    Figure 2:Immunofluorescence analysis of A431 cells using PKHD1 Rat mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

  • FC - PKHD1 Antibody AO2006a
    Figure 3: Flow cytometric analysis of Hela cells using PKHD1 Rat mAb (green) and negative control (red).
  • IHC - PKHD1 Antibody AO2006a
    Figure 4: Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using PKHD1 Rat mAb with DAB staining.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 8G12A1
Isotype IgG1
Calculated MW 445kDa
Description Mutations in the PKHD1 gene, which encodes fibrocystin, cause autosomal recessive polycystic kidney disease (ARPKD). Unfortunately, the lack of specific antibodies to the mouse protein impairs the study of splicing, post-translational processing, shedding, and temporal and spatial expression of endogenous fibrocystin at the cellular and subcellular level.
Immunogen Purified recombinant fragment of mouse PKHD1 (AA: 3878-4060) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Dilution E~~1/10000
WB~~1/500 - 1/2000
IF~~1/200 - 1/1000
FC~~1/200 - 1/400
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPKHD1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Citations (0)

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J Am Soc Nephrol. 2011 Dec;22(12):2266-77.Am J Pathol. 2008 Feb;172(2):417-29.

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$ 385.00
Cat# AO2006a
(40 western blots)
Availability: 7-10 days
Bulk Size
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