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EZH2 Antibody

Purified Mouse Monoclonal Antibody

  • E - EZH2 Antibody AO2015a

    Figure 1:Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - EZH2 Antibody AO2015a

    Figure 2:Western blot analysis using EZH2 mAb against human EZH2 (AA: 1-194) recombinant protein. (Expected MW is 48.5 kDa)

  • WB - EZH2 Antibody AO2015a

    Figure 3:Western blot analysis using EZH2 mAb against HEK293 (1) and EZH2 (AA: 1-194)-hIgGFc transfected HEK293 (2) cell lysate.

  • FC - EZH2 Antibody AO2015a

    Figure 4:Flow cytometric analysis of Hela cells using EZH2 mouse mAb (green) and negative control (red).

  • IHC - EZH2 Antibody AO2015a

    Figure 5:Immunohistochemical analysis of paraffin-embedded lung cancer tissues using EZH2 mouse mAb with DAB staining.

  • IHC - EZH2 Antibody AO2015a

    Figure 6:Immunohistochemical analysis of paraffin-embedded colon cancer tissues using EZH2 mouse mAb with DAB staining.

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q15910
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 6G4F4
Isotype IgG1
Calculated MW 85.4kDa
Description This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. This protein may play a role in the hematopoietic and central nervous systems. Multiple alternatively splcied transcript variants encoding distinct isoforms have been identified for this gene.
Immunogen Purified recombinant fragment of human EZH2 (AA: 1-194) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 2146
Other Names Histone-lysine N-methyltransferase EZH2,, ENX-1, Enhancer of zeste homolog 2, Lysine N-methyltransferase 6, EZH2, KMT6
Dilution E~~1/10000
WB~~1/500 - 1/2000
FC~~1/200 - 1/400
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsEZH2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name EZH2
Synonyms KMT6
Function Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' (H3K9me) and 'Lys- 27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Compared to EZH2-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1, CDKN2A and retinoic acid target genes. EZH2 can also methylate non-histone proteins such as the transcription factor GATA4 and the nuclear receptor RORA. Regulates the circadian clock via histone methylation at the promoter of the circadian genes. Essential for the CRY1/2-mediated repression of the transcriptional activation of PER1/2 by the CLOCK-ARNTL/BMAL1 heterodimer; involved in the di and trimethylation of 'Lys-27' of histone H3 on PER1/2 promoters which is necessary for the CRY1/2 proteins to inhibit transcription.
Cellular Location Nucleus
Tissue Location Expressed in many tissues. Overexpressed in numerous tumor types including carcinomas of the breast, colon, larynx, lymphoma and testis.
Research Areas
Citations (0)

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Int J Gynecol Cancer. 2013 Jul;23(6):997-1005.Cell Cycle. 2013 Jul 1;12(13):2113-9.

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$ 385.00
Cat# AO2015a
(40 western blots)
Availability: 7-10 days
Bulk Size
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