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VAV2 Antibody

Purified Mouse Monoclonal Antibody

  • E - VAV2 Antibody AO2020a

    Figure 1:Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - VAV2 Antibody AO2020a

    Figure 2:Western blot analysis using VAV2 mAb against human VAV2 (AA: 552-868) recombinant protein. (Expected MW is *** kDa)

  • WB - VAV2 Antibody AO2020a

    Figure 3:Western blot analysis using VAV2 mAb against HEK293 (1) and VAV2 (AA: 552-868)-hIgGFc transfected HEK293 (2) cell lysate.

  • FC - VAV2 Antibody AO2020a

    Figure 4:Flow cytometric analysis of Hela cells using VAV2 mouse mAb (green) and negative control (red).

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P52735
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 3F5D3
Isotype IgG1
Calculated MW 101.3kDa
Description VAV2 is the second member of the VAV guanine nucleotide exchange factor family of oncogenes. Unlike VAV1, which is expressed exclusively in hematopoietic cells, VAV2 transcripts were found in most tissues. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Immunogen Purified recombinant fragment of human VAV2 (AA: 552-868) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 7410
Other Names Guanine nucleotide exchange factor VAV2, VAV-2, VAV2
Dilution E~~1/10000
WB~~1/500 - 1/2000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsVAV2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name VAV2
Function Guanine nucleotide exchange factor for the Rho family of Ras-related GTPases. Plays an important role in angiogenesis. Its recruitment by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly (By similarity).
Tissue Location Widely expressed.
Research Areas
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J Cell Biol. 2001 Jul 9;154(1):177-86.Oncogene. 2010 Apr 29;29(17):2528-39.

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$ 385.00
Cat# AO2020a
(40 western blots)
Availability: 7-10 days
Bulk Size
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