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MGMT Antibody

Purified Mouse Monoclonal Antibody

     
  • E - MGMT Antibody AO2041a

    Figure 2:Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - MGMT Antibody AO2041a

    Figure 2:Western blot analysis using MGMT mAb against human MGMT (AA: 32-210) recombinant protein. (Expected MW is 45.2 kDa)

  • WB - MGMT Antibody AO2041a

    Figure 3:Western blot analysis using MGMT mAb against HEK293 (1) and MGMT (AA: 32-210)-hIgGFc transfected HEK293 (2) cell lysate.

  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P16455
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 1A11A8
Isotype IgG1
Calculated MW 21.6kDa
Description MGMT involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA.Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated
Immunogen Purified recombinant fragment of human MGMT (AA: 32-210) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 4255
Other Names Methylated-DNA--protein-cysteine methyltransferase, 2.1.1.63, 6-O-methylguanine-DNA methyltransferase, MGMT, O-6-methylguanine-DNA-alkyltransferase, MGMT
Dilution E~~1/10000
WB~~1/500 - 1/2000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMGMT Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MGMT
Function Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.
Cellular Location Nucleus.
Citations (0)

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References

Folia Neuropathol. 2013;51(4):275-82.Fam Cancer. 2013 Sep;12(3):449-58.

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$ 385.00
Cat# AO2041a
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