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TTR Antibody

Purified Mouse Monoclonal Antibody

  • E - TTR Antibody AO2046a

    Figure 1:Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - TTR Antibody AO2046a

    Figure 2:Western blot analysis using TTR mAb against human TTR (AA: 1-147) recombinant protein. (Expected MW is 45.8 kDa)

  • WB - TTR Antibody AO2046a

    Figure 3:Western blot analysis using TTR mAb against HEK293 (1) and TTR (AA: 1-147)-hIgGFc transfected HEK293 (2) cell lysate.

  • IF - TTR Antibody AO2046a

    Figure 4:Immunofluorescence analysis of A549 cells using TTR mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

  • IF - TTR Antibody AO2046a

    Figure 5:Immunofluorescence analysis of MCF-7 cells using TTR mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

  • FC - TTR Antibody AO2046a

    Figure 6:Flow cytometric analysis of A549 cells using TTR mouse mAb (green) and negative control (red).

  • IHC - TTR Antibody AO2046a

    Figure 7:Immunohistochemical analysis of paraffin-embedded liver cancer tissues using TTR mouse mAb with DAB staining.

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P02766
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 2E10C5
Isotype IgG1
Calculated MW 15.9kDa
Description This gene encodes transthyretin, one of the three prealbumins including alpha-1-antitrypsin, transthyretin and orosomucoid. Transthyretin is a carrier protein; it transports thyroid hormones in the plasma and cerebrospinal fluid, and also transports retinol (vitamin A) in the plasma. The protein consists of a tetramer of identical subunits. More than 80 different mutations in this gene have been reported; most mutations are related to amyloid deposition, affecting predominantly peripheral nerve and/or the heart, and a small portion of the gene mutations is non-amyloidogenic. The diseases caused by mutations include amyloidotic polyneuropathy, euthyroid hyperthyroxinaemia, amyloidotic vitreous opacities, cardiomyopathy, oculoleptomeningeal amyloidosis, meningocerebrovascular amyloidosis, carpal tunnel syndrome, etc. [provided by RefSeq, Jan 2009]
Immunogen Purified recombinant fragment of human TTR (AA: 1-147) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 7276
Other Names Transthyretin, ATTR, Prealbumin, TBPA, TTR, PALB
Dilution E~~1/10000
WB~~1/500 - 1/2000
IF~~1/200 - 1/1000
FC~~1/200 - 1/400
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTTR Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TTR
Synonyms PALB
Function Thyroid hormone-binding protein. Probably transports thyroxine from the bloodstream to the brain.
Cellular Location Secreted. Cytoplasm.
Tissue Location Detected in serum and cerebrospinal fluid (at protein level). Highly expressed in choroid plexus epithelial cells. Detected in retina pigment epithelium and liver
Research Areas
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J Biol Chem. 2013 Nov 1;288(44):31752-60.Clin Exp Rheumatol. 2013 May-Jun;31(3):394-9.

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$ 385.00
Cat# AO2046a
(40 western blots)
Availability: 7-10 days
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