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EMD Antibody

Purified Mouse Monoclonal Antibody

     
  • E - EMD Antibody AO2052a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

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  • WB - EMD Antibody AO2052a

    Figure 2:Western blot analysis using EMD mAb against human EMD (AA: 1-222) recombinant protein. (Expected MW is 51.1 kDa)

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  • WB - EMD Antibody AO2052a

    Figure 3:Western blot analysis using EMD mAb against HEK293 (1) and EMD (AA: 1-222)-hIgGFc transfected HEK293 (2) cell lysate.

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  • IF - EMD Antibody AO2052a

    Figure 4:Immunofluorescence analysis of A549 cells using EMD mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

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  • IF - EMD Antibody AO2052a

    Figure 5:Immunofluorescence analysis of Hela cells using EMD mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

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  • FC - EMD Antibody AO2052a

    Figure 6:Flow cytometric analysis of K562 cells using EMD mouse mAb (green) and negative control (red).

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  • FC - EMD Antibody AO2052a

    Figure 7:Flow cytometric analysis of A549 cells using EMD mouse mAb (green) and negative control (red).

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  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC, E
Primary Accession P50402
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 8F5G2
Isotype IgG1
Calculated MW 29kDa
Description Emerin is a serine-rich nuclear membrane protein and a member of the nuclear lamina-associated protein family. It mediates membrane anchorage to the cytoskeleton. Dreifuss-Emery muscular dystrophy is an X-linked inherited degenerative myopathy resulting from mutation in the emerin gene.
Immunogen Purified recombinant fragment of human *** (AA: 1-222) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 2010
Other Names Emerin, EMD, EDMD, STA
Dilution E~~1/10000
WB~~1/500 - 1/2000
IF~~1/200 - 1/1000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsEMD Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name EMD
Synonyms EDMD, STA
Function Stabilizes and promotes the formation of a nuclear actin cortical network. Stimulates actin polymerization in vitro by binding and stabilizing the pointed end of growing filaments. Inhibits beta- catenin activity by preventing its accumulation in the nucleus. Acts by influencing the nuclear accumulation of beta-catenin through a CRM1- dependent export pathway. Links centrosomes to the nuclear envelope via a microtubule association. Required for proper localization of non- farnesylated prelamin-A/C. Together with NEMP1, contributes to nuclear envelope stiffness in germ cells (PubMed:32923640). EMD and BAF are cooperative cofactors of HIV-1 infection. Association of EMD with the viral DNA requires the presence of BAF and viral integrase. The association of viral DNA with chromatin requires the presence of BAF and EMD.
Cellular Location Nucleus inner membrane; Single-pass membrane protein; Nucleoplasmic side. Nucleus outer membrane. Note=Colocalized with BANF1 at the central region of the assembling nuclear rim, near spindle-attachment sites. The accumulation of different intermediates of prelamin-A/C (non-farnesylated or carboxymethylated farnesylated prelamin-A/C) in fibroblasts modify its localization in the nucleus
Tissue Location Skeletal muscle, heart, colon, testis, ovary and pancreas
Research Areas
Citations (0)
citation

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References

1.Histopathology. 2009 Apr;54(5):571-9.2.J Cell Biol. 2007 Sep 10;178(6):897-904.

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$ 385.00
Cat# AO2052a
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