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CSF3 Antibody

Purified Mouse Monoclonal Antibody

  • E - CSF3 Antibody AO2054a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - CSF3 Antibody AO2054a

    Figure 2:Western blot analysis using CSF3 mAb against human CSF3 (AA: 1-207) recombinant protein. (Expected MW is 48.2 kDa)

  • WB - CSF3 Antibody AO2054a

    Figure 3:Western blot analysis using CSF3 mAb against HEK293 (1) and CSF3 (AA: 1-207)-hIgGFc transfected HEK293 (2) cell lysate.

  • IF - CSF3 Antibody AO2054a

    Figure 4:Immunofluorescence analysis of A549 cells using CSF3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

  • IF - CSF3 Antibody AO2054a

    Figure 5:Immunofluorescence analysis of Hela cells using CSF3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

  • FC - CSF3 Antibody AO2054a

    Figure 6:Flow cytometric analysis of A549 cells using CSF3 mouse mAb (green) and negative control (red).

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P09919
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 8G5F7
Isotype IgG1
Calculated MW 22.3kDa
Description The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes. The active protein is found extracellularly. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, May 2010]
Immunogen Purified recombinant fragment of human CSF3 (AA: 1-207) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 1440
Other Names Granulocyte colony-stimulating factor, G-CSF, Pluripoietin, Filgrastim, Lenograstim, CSF3, C17orf33, GCSF
Dilution E~~1/10000
WB~~1/500 - 1/2000
IF~~1/200 - 1/1000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCSF3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CSF3
Synonyms C17orf33, GCSF
Function Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.
Cellular Location Secreted.
Research Areas
Citations (0)

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1.Biomarkers. 2012 Jun;17(4):319-24.2.Eur J Immunol. 2010 Nov;40(11):3097-106.

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$ 385.00
Cat# AO2054a
(40 western blots)
Availability: 7-10 days
Bulk Size
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