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ANXA5 Antibody

Purified Mouse Monoclonal Antibody

  • E - ANXA5 Antibody AO2058a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - ANXA5 Antibody AO2058a

    Figure 2:Western blot analysis using ANXA5 mAb against human ANXA5 (AA: 160-320) recombinant protein. (Expected MW is 44.1 kDa)

  • WB - ANXA5 Antibody AO2058a

    Figure 3:Western blot analysis using ANXA5 mAb against HEK293 (1) and ANXA5 (AA: 160-320)-hIgGFc transfected HEK293 (2) cell lysate.

  • WB - ANXA5 Antibody AO2058a

    Figure 4:Western blot analysis using ANXA5 mouse mAb against HepG2 (1), PNAC-1 (2), NIH/3T3 (3), Hela (4), MCF-7 (5), and A431 (6) cell lysate.

  • IF - ANXA5 Antibody AO2058a

    Figure 5:Immunofluorescence analysis of Hela cells using ANXA5 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

  • FC - ANXA5 Antibody AO2058a

    Figure 6:Flow cytometric analysis of Hela cells using ANXA5 mouse mAb (green) and negative control (red).

  • FC - ANXA5 Antibody AO2058a

    Figure 7:Flow cytometric analysis of MCF-7 cells using ANXA5 mouse mAb (green) and negative control (red).

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P08758
Reactivity Human, Mouse
Host Mouse
Clonality Monoclonal
Clone Names 3B9B7
Isotype IgG1
Calculated MW 36kDa
Description The protein encoded by this gene belongs to the annexin family of calcium-dependent phospholipid binding proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII. The gene spans 29 kb containing 13 exons, and encodes a single transcript of approximately 1.6 kb and a protein product with a molecular weight of about 35 kDa.
Immunogen Purified recombinant fragment of human ANXA5 (AA: 160-320) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 308
Other Names Annexin A5, Anchorin CII, Annexin V, Annexin-5, Calphobindin I, CBP-I, Endonexin II, Lipocortin V, Placental anticoagulant protein 4, PP4, Placental anticoagulant protein I, PAP-I, Thromboplastin inhibitor, Vascular anticoagulant-alpha, VAC-alpha, ANXA5, ANX5, ENX2, PP4
Dilution E~~1/10000
WB~~1/500 - 1/2000
IF~~1/200 - 1/1000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsANXA5 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ANXA5
Synonyms ANX5, ENX2, PP4
Function This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
Research Areas
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1.Klin Lab Diagn. 2013 Apr;(4):9-10.2.Mech Ageing Dev. 2012 Jul;133(7):508-22.

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$ 385.00
Cat# AO2058a
(40 western blots)
Availability: 7-10 days
Bulk Size
Seasonal Special on Bulk Order
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