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NFKBIA Antibody

Purified Mouse Monoclonal Antibody

  • E - NFKBIA Antibody AO2059a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - NFKBIA Antibody AO2059a

    Figure 2:Western blot analysis using NFKBIA mAb against human NFKBIA (AA: 150-291) recombinant protein. (Expected MW is 42.4 kDa)

  • WB - NFKBIA Antibody AO2059a

    Figure 3:Western blot analysis using NFKBIA mAb against HEK293 (1) and NFKBIA (AA: 150-291)-hIgGFc transfected HEK293 (2) cell lysate.

  • IF - NFKBIA Antibody AO2059a

    Figure 4:Immunofluorescence analysis of Hela cells using NFKBIA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

  • IF - NFKBIA Antibody AO2059a

    Figure 5:Immunofluorescence analysis of MCF-7 cells using NFKBIA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

  • FC - NFKBIA Antibody AO2059a

    Figure 6:Flow cytometric analysis of A549 cells using NFKBIA mouse mAb (green) and negative control (red).

  • IHC - NFKBIA Antibody AO2059a

    Figure 7:Immunohistochemical analysis of paraffin-embedded liver cancer tissues using NFKBIA mouse mAb with DAB staining.

  • IHC - NFKBIA Antibody AO2059a

    Figure 8:Immunohistochemical analysis of paraffin-embedded brain tissues using NFKBIA mouse mAb with DAB staining.

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P25963
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 4D4C4
Isotype IgG1
Calculated MW 35.6kDa
Description This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease.
Immunogen Purified recombinant fragment of human NFKBIA (AA: 150-291) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 4792
Other Names NF-kappa-B inhibitor alpha, I-kappa-B-alpha, IkB-alpha, IkappaBalpha, Major histocompatibility complex enhancer-binding protein MAD3, NFKBIA, IKBA, MAD3, NFKBI
Dilution E~~1/10000
WB~~1/500 - 1/2000
IF~~1/200 - 1/1000
FC~~1/200 - 1/400
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNFKBIA Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms IKBA, MAD3, NFKBI
Function Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.
Cellular Location Cytoplasm. Nucleus. Note=Shuttles between the nucleus and the cytoplasm by a nuclear localization signal (NLS) and a CRM1-dependent nuclear export.
Research Areas
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1.Mol Cancer. 2013 Dec 11;12:160.2.Acta Med Okayama. 2013;67(1):19-24.

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$ 385.00
Cat# AO2059a
(40 western blots)
Availability: 7-10 days
Bulk Size
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