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EZR Antibody

Purified Mouse Monoclonal Antibody

  • E - EZR Antibody AO2061a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - EZR Antibody AO2061a

    Figure 2:Western blot analysis using EZR mAb against human EZR (AA: 292-464) recombinant protein. (Expected MW is 47.1 kDa)

  • WB - EZR Antibody AO2061a

    Figure 3:Western blot analysis using EZR mAb against HEK293 (1) and EZR (AA: 292-464)-hIgGFc transfected HEK293 (2) cell lysate.

  • WB - EZR Antibody AO2061a

    Figure 4:Western blot analysis using EZR mouse mAb against MCF-7 (1), Hela (2), A431 (3), Hek293 (4), SK-N-SH (5), Jurkat (6), HepG2 (7), NIH/3T3 (8), and Cos7 (9) cell lysate.

  • IF - EZR Antibody AO2061a

    Figure 5:Immunofluorescence analysis of A549 cells using EZR mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

  • FC - EZR Antibody AO2061a

    Figure 6:Flow cytometric analysis of MCF-7 cells using EZR mouse mAb (green) and negative control (red).

  • FC - EZR Antibody AO2061a

    Figure 7:Flow cytometric analysis of Hela cells using EZR mouse mAb (green) and negative control (red).

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P15311
Reactivity Human, Mouse, Monkey
Host Mouse
Clonality Monoclonal
Clone Names 6F1A9
Isotype IgG1
Calculated MW 69.4kDa
Description The cytoplasmic peripheral membrane protein encoded by this gene functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein serves as an intermediate between the plasma membrane and the actin cytoskeleton. This protein plays a key role in cell surface structure adhesion, migration and organization, and it has been implicated in various human cancers. A pseudogene located on chromosome 3 has been identified for this gene. Alternatively spliced variants have also been described for this gene.
Immunogen Purified recombinant fragment of human EZR (AA: 292-464) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 7430
Other Names Ezrin, Cytovillin, Villin-2, p81, EZR, VIL2
Dilution E~~1/10000
WB~~1/500 - 1/2000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsEZR Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name EZR
Synonyms VIL2
Function Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis.
Cellular Location Apical cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection. Cell projection, microvillus membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, ruffle membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cell cortex. Cytoplasm, cytoskeleton. Note=Localization to the apical membrane of parietal cells depends on the interaction with MPP5 Localizes to cell extensions and peripheral processes of astrocytes (By similarity). Microvillar peripheral membrane protein (cytoplasmic side).
Tissue Location Expressed in cerebral cortex, basal ganglia, hippocampus, hypophysis, and optic nerve. Weakly expressed in brain stem and diencephalon. Stronger expression was detected in gray matter of frontal lobe compared to white matter (at protein level). Component of the microvilli of intestinal epithelial cells. Preferentially expressed in astrocytes of hippocampus, frontal cortex, thalamus, parahippocampal cortex, amygdala, insula, and corpus callosum. Not detected in neurons in most tissues studied.
Research Areas
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1.BMC Cancer. 2013 Nov 4;13:520.2.Cell Oncol (Dordr). 2013 Dec;36(6):485-91.

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$ 385.00
Cat# AO2061a
(40 western blots)
Availability: 7-10 days
Bulk Size
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