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CBX4 Antibody

Purified Mouse Monoclonal Antibody

  • E - CBX4 Antibody AO2074a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - CBX4 Antibody AO2074a

    Figure 2:Western blot analysis using CBX4 mAb against human CBX4 (AA: 397-514) recombinant protein. (Expected MW is 43.3 kDa)

  • WB - CBX4 Antibody AO2074a

    Figure 3:Western blot analysis using CBX4 mAb against HEK293 (1) and CBX4 (AA: 397-514)-hIgGFc transfected HEK293 (2) cell lysate.

  • FC - CBX4 Antibody AO2074a

    Figure 4:Flow cytometric analysis of Hela cells using CBX4 mouse mAb (green) and negative control (red).

  • IHC - CBX4 Antibody AO2074a

    Figure 5:Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using CBX4 mouse mAb with DAB staining.

  • IHC - CBX4 Antibody AO2074a

    Figure 6:Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using CBX4 mouse mAb with DAB staining.

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O00257
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 6C5G3
Isotype IgG1
Calculated MW 61.4kDa
Description CBX4 (chromobox homolog 4) is a protein-coding gene. Diseases associated with CBX4 include brain cancer. GO annotations related to this gene include chromatin binding and transcription corepressor activity. An important paralog of this gene is CBX8.
Immunogen Purified recombinant fragment of human CBX4 (AA: 397-514) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 8535
Other Names E3 SUMO-protein ligase CBX4, 6.3.2.-, Chromobox protein homolog 4, Polycomb 2 homolog, Pc2, hPc2, CBX4
Dilution E~~1/10000
WB~~1/500 - 1/2000
FC~~1/200 - 1/400
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCBX4 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CBX4
Function E3 SUMO-protein ligase which facilitates SUMO1 conjugation by UBE2I. Involved in the sumoylation of HNRNPK, a p53/TP53 transcriptional coactivator, hence indirectly regulates p53/TP53 transcriptional activation resulting in p21/CDKN1A expression. Monosumoylates ZNF131.
Cellular Location Nucleus. Nucleus speckle. Note=Localization to nuclear polycomb bodies is required for ZNF131 sumoylation
Tissue Location Ubiquitous.
Citations (0)

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1.Cell Stem Cell. 2011 Sep 2;9(3):233-46.2.Ann Surg Oncol. 2013 Dec;20 Suppl 3:S684-92.

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$ 385.00
Cat# AO2074a
(40 western blots)
Availability: 7-10 days
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