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CIDEC Antibody

Purified Mouse Monoclonal Antibody

     
  • E - CIDEC Antibody AO2077a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

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  • WB - CIDEC Antibody AO2077a

    Figure 2:Western blot analysis using CIDEC mAb against human CIDEC (AA: 53-141) recombinant protein. (Expected MW is 36 kDa)

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  • WB - CIDEC Antibody AO2077a

    Figure 3:Western blot analysis using CIDEC mAb against HEK293 (1) and CIDEC (AA: 53-141)-hIgGFc transfected HEK293 (2) cell lysate.

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  • WB - CIDEC Antibody AO2077a

    Figure 4:Western blot analysis using CIDEC mouse mAb against HEK293 (1), A431 (2), and HCT116 (3) cell lysate.

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  • IF - CIDEC Antibody AO2077a

    Figure 5:Immunofluorescence analysis of Hela cells using CIDEC mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

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  • FC - CIDEC Antibody AO2077a

    Figure 6:Flow cytometric analysis of Hela cells using CIDEC mouse mAb (green) and negative control (red).

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  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC, E
Primary Accession Q96AQ7
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 7C12F11
Isotype IgG1
Calculated MW 26.8kDa
Description This gene encodes a member of the cell death-inducing DNA fragmentation factor-like effector family. Members of this family play important roles in apoptosis. The encoded protein promotes lipid droplet formation in adipocytes and may mediate adipocyte apoptosis. This gene is regulated by insulin and its expression is positively correlated with insulin sensitivity. Mutations in this gene may contribute to insulin resistant diabetes. A pseudogene of this gene is located on the short arm of chromosome 3. Alternatively spliced transcript variants that encode different isoforms have been observed for this gene.
Immunogen Purified recombinant fragment of human CIDEC (AA: 53-141) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 63924
Other Names Cell death activator CIDE-3, Cell death-inducing DFFA-like effector protein C, Fat-specific protein FSP27 homolog, CIDEC, FSP27
Dilution E~~1/10000
WB~~1/500 - 1/2000
IF~~1/200 - 1/1000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCIDEC Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CIDEC {ECO:0000303|PubMed:20049731, ECO:0000312|HGNC:HGNC:24229}
Function Lipid transferase specifically expressed in white adipose tissue, which promotes unilocular lipid droplet formation by mediating lipid droplet fusion (PubMed:18334488, PubMed:19843876, PubMed:20049731, PubMed:23399566, PubMed:30361435). Lipid droplet fusion promotes their enlargement, restricting lipolysis and favoring lipid storage (PubMed:18334488, PubMed:19843876, PubMed:20049731, PubMed:23399566). Localizes on the lipid droplet surface, at focal contact sites between lipid droplets, and mediates atypical lipid droplet fusion by undergoing liquid-liquid phase separation (LLPS) and promoting directional net neutral lipid transfer from the smaller to larger lipid droplets (PubMed:18334488, PubMed:19843876, PubMed:20049731, PubMed:23399566). The transfer direction may be driven by the internal pressure difference between the contacting lipid droplet pair (PubMed:18334488, PubMed:19843876, PubMed:20049731, PubMed:23399566). Its role in neutral lipid transfer and lipid droplet enlargement is activated by the interaction with PLIN1 (PubMed:23399566). May also act as a CEBPB coactivator in the white adipose tissue to control the expression of a subset of CEBPB downstream target genes, including SOCS1, SOCS3, TGFB1, TGFBR1, ID2 and XDH (By similarity). When overexpressed in preadipocytes, induces apoptosis or increases cell susceptibility to apoptosis induced by serum deprivation or TGFB treatment (PubMed:12429024).
Cellular Location Lipid droplet. Endoplasmic reticulum {ECO:0000250|UniProtKB:P56198}. Nucleus {ECO:0000250|UniProtKB:P56198} Note=Diffuses quickly on lipid droplet surface, but becomes trapped and clustered at lipid droplet contact sites, thereby enabling its rapid enrichment at lipid droplet contact sites {ECO:0000250|UniProtKB:P56198}
Tissue Location Expressed mainly in adipose tissue, small intestine, heart, colon and stomach and, at lower levels, in brain, kidney and liver.
Research Areas
Citations (0)
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References

1.Obesity (Silver Spring). 2010 Feb;18(2):417-9.2.EMBO Mol Med. 2009 Aug;1(5):280-7.

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$ 385.00
Cat# AO2077a
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