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HDAC9 Antibody

Purified Mouse Monoclonal Antibody

  • E - HDAC9 Antibody AO2081a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - HDAC9 Antibody AO2081a

    Figure 2:Western blot analysis using HDAC9 mAb against human HDAC9 (AA: 343-569) recombinant protein. (Expected MW is 50.5 kDa)

  • WB - HDAC9 Antibody AO2081a

    Figure 3:Western blot analysis using HDAC9 mAb against HEK293 (1) and HDAC9 (AA:343-569)-hIgGFc transfected HEK293 (2) cell lysate.

  • FC - HDAC9 Antibody AO2081a

    Figure 4:Flow cytometric analysis of Hela cells using HDAC9 mouse mAb (green) and negative control (red).

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9UKV0
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 2B7C4
Isotype IgG1
Calculated MW 111.3kDa
Description Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene has sequence homology to members of the histone deacetylase family. This gene is orthologous to the Xenopus and mouse MITR genes. The MITR protein lacks the histone deacetylase catalytic domain. It represses MEF2 activity through recruitment of multicomponent corepressor complexes that include CtBP and HDACs. This encoded protein may play a role in hematopoiesis. Multiple alternatively spliced transcripts have been described for this gene but the full-length nature of some of them has not been determined.
Immunogen Purified recombinant fragment of human HDAC9 (AA: 343-569) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 9734
Other Names Histone deacetylase 9, HD9,, Histone deacetylase 7B, HD7, HD7b, Histone deacetylase-related protein, MEF2-interacting transcription repressor MITR, HDAC9, HDAC7, HDAC7B, HDRP, KIAA0744, MITR
Dilution E~~1/10000
WB~~1/500 - 1/2000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHDAC9 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HDAC9
Synonyms HDAC7, HDAC7B, HDRP, KIAA0744, MITR
Function Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Represses MEF2-dependent transcription.
Cellular Location Nucleus.
Tissue Location Broadly expressed, with highest levels in brain, heart, muscle and testis. Isoform 3 is present in human bladder carcinoma cells (at protein level)
Research Areas
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1.Int J Clin Exp Pathol. 2013 Dec 15;7(1):213-20.2.J Biol Chem. 2011 Jan 21;286(3):2343-53.

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$ 385.00
Cat# AO2081a
(40 western blots)
Availability: 7-10 days
Bulk Size
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