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CBX3 Antibody

Purified Mouse Monoclonal Antibody

  • E - CBX3 Antibody AO2082a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - CBX3 Antibody AO2082a

    Figure 2:Western blot analysis using CBX3 mAb against human CBX3 (AA: 1-183) recombinant protein. (Expected MW is 46.8 kDa)

  • WB - CBX3 Antibody AO2082a

    Figure 3:Western blot analysis using CBX3 mAb against HEK293 (1) and CBX3 (AA: 1-183)-hIgGFc transfected HEK293 (2) cell lysate.

  • FC - CBX3 Antibody AO2082a

    Figure 4:Flow cytometric analysis of A431 cells using CBX3 mouse mAb (green) and negative control (red).

  • IHC - CBX3 Antibody AO2082a

    Figure 5:Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using CBX3 mouse mAb with DAB staining.

  • IHC - CBX3 Antibody AO2082a

    Figure 6:Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using CBX3 mouse mAb with DAB staining.

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q13185
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 6D1C4
Isotype IgG1
Calculated MW 20.8kDa
Description At the nuclear envelope, the nuclear lamina and heterochromatin are adjacent to the inner nuclear membrane. The protein encoded by this gene binds DNA and is a component of heterochromatin. This protein also can bind lamin B receptor, an integral membrane protein found in the inner nuclear membrane. The dual binding functions of the encoded protein may explain the association of heterochromatin with the inner nuclear membrane. This protein binds histone H3 tails methylated at Lys-9 sites. This protein is also recruited to sites of ultraviolet-induced DNA damage and double-strand breaks. Two transcript variants encoding the same protein but differing in the 5' UTR, have been found for this gene.
Immunogen Purified recombinant fragment of human CBX3 (AA: 1-183) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 11335
Other Names Chromobox protein homolog 3, HECH, Heterochromatin protein 1 homolog gamma, HP1 gamma, Modifier 2 protein, CBX3
Dilution E~~1/10000
WB~~1/500 - 1/2000
FC~~1/200 - 1/400
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCBX3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CBX3
Function Seems to be involved in transcriptional silencing in heterochromatin-like complexes. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. May contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR). Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins. Contributes to the conversion of local chromatin to a heterochromatin-like repressive state through H3 'Lys-9' trimethylation, mediates the recruitment of the mehtyltransferases SUV39H1 and/or SUV39H2 by the PER complex to the E-box elements of the circadian target genes such as PER2 itself or PER1.
Cellular Location Nucleus. Note=Associates with euchromatin and is largely excluded from constitutive heterochromatin. May be associated with microtubules and mitotic poles during mitosis (Potential).
Citations (0)

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1.BMC Cancer. 2013 Mar 23;13:148.2.PLoS One. 2012;7(8):e41401.

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$ 385.00
Cat# AO2082a
(40 western blots)
Availability: 7-10 days
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