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TARDBP Antibody

Purified Mouse Monoclonal Antibody

     
  • E - TARDBP Antibody AO2088a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

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  • WB - TARDBP Antibody AO2088a

    Figure 2:Western blot analysis using TARDBP mAb against human TARDBP (AA: 126-260) recombinant protein. (Expected MW is 41.5 kDa)

    detail
  • WB - TARDBP Antibody AO2088a

    Figure 3:Western blot analysis using TARDBP mAb against HEK293 (1) and TARDBP (AA: 126-260)-hIgGFc transfected HEK293 (2) cell lysate.

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  • FC - TARDBP Antibody AO2088a

    Figure 4:Flow cytometric analysis of Hela cells using TARDBP mouse mAb (green) and negative control (red).

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  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, FC, E
Primary Accession Q13148
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 7F9A6
Isotype IgG1
Calculated MW 44.7kDa
Description HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. In addition, this protein regulates alternate splicing of the CFTR gene. A similar pseudogene is present on chromosome 20.
Immunogen Purified recombinant fragment of human TARDBP (AA: 126-260) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 23435
Other Names TAR DNA-binding protein 43, TDP-43, TARDBP, TDP43
Dilution E~~1/10000
WB~~1/500 - 1/2000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTARDBP Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TARDBP {ECO:0000303|PubMed:18396105, ECO:0000312|HGNC:HGNC:11571}
Function RNA-binding protein that is involved in various steps of RNA biogenesis and processing (PubMed:23519609). Preferentially binds, via its two RNA recognition motifs RRM1 and RRM2, to GU-repeats on RNA molecules predominantly localized within long introns and in the 3'UTR of mRNAs (PubMed:23519609, PubMed:24240615, PubMed:24464995). In turn, regulates the splicing of many non-coding and protein-coding RNAs including proteins involved in neuronal survival, as well as mRNAs that encode proteins relevant for neurodegenerative diseases (PubMed:21358640, PubMed:29438978). Plays a role in maintaining mitochondrial homeostasis by regulating the processing of mitochondrial transcripts (PubMed:28794432). Regulates also mRNA stability by recruiting CNOT7/CAF1 deadenylase on mRNA 3'UTR leading to poly(A) tail deadenylation and thus shortening (PubMed:30520513). In response to oxidative insult, associates with stalled ribosomes localized to stress granules (SGs) and contributes to cell survival (PubMed:23398327, PubMed:19765185). Participates also in the normal skeletal muscle formation and regeneration, forming cytoplasmic myo-granules and binding mRNAs that encode sarcomeric proteins (PubMed:30464263). Plays a role in the maintenance of the circadian clock periodicity via stabilization of the CRY1 and CRY2 proteins in a FBXL3-dependent manner (PubMed:27123980). Negatively regulates the expression of CDK6 (PubMed:19760257). Regulates the expression of HDAC6, ATG7 and VCP in a PPIA/CYPA-dependent manner (PubMed:25678563).
Cellular Location Nucleus. Cytoplasm. Cytoplasm, Stress granule Mitochondrion. Note=Continuously travels in and out of the nucleus (PubMed:18957508). Localizes to stress granules in response to oxidative stress (PubMed:19765185). A small subset localizes in mitochondria (PubMed:28794432).
Tissue Location Ubiquitously expressed. In particular, expression is high in pancreas, placenta, lung, genital tract and spleen
Research Areas
Citations (0)
citation

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References

1.Exp Cell Res. 2013 Aug 1;319(13):1998-2005.2.PLoS One. 2013 May 30;8(5):e64002.

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$ 385.00
Cat# AO2088a
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