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RAD18 Antibody
Purified Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, FC, E |
|---|---|
| Primary Accession | Q9NS91 |
| Reactivity | Human |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone Names | 2G8C10 |
| Isotype | IgG1 |
| Calculated MW | 56.2kDa |
| Description | The protein encoded by this gene is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens. |
| Immunogen | Purified recombinant fragment of human RAD18 (AA: 332-430) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
| Gene ID | 56852 |
|---|---|
| Other Names | E3 ubiquitin-protein ligase RAD18, 6.3.2.-, Postreplication repair protein RAD18, hHR18, hRAD18, RING finger protein 73, RAD18, RNF73 |
| Dilution | E~~1/10000 WB~~1/500 - 1/2000 FC~~1/200 - 1/400 |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | RAD18 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | RAD18 |
|---|---|
| Synonyms | RNF73 |
| Function | E3 ubiquitin-protein ligase involved in postreplication repair of UV-damaged DNA. Postreplication repair functions in gap- filling of a daughter strand on replication of damaged DNA. Associates to the E2 ubiquitin conjugating enzyme UBE2B to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA- associated PCNA on 'Lys-164'. Has ssDNA binding activity. |
| Cellular Location | Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Associates with chromatin (PubMed:25931565). Colocalizes with SLF1 in the nucleus and to centrosomes (PubMed:15632077). Relocalizes with SLF1 to nuclear foci in response to DNA damage (PubMed:22036607). Accumulates with the SLF1- SLF2 and SMC5-SMC6 complexes at replication-coupled DNA interstrand repair and DNA double-strand breaks (DSBs) sites on chromatin in a ubiquitin-dependent manner (PubMed:25931565) |
Research Areas
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Application Protocols
Provided below are standard protocols that you may find useful for product applications.
References
1.Pigment Cell Melanoma Res. 2012 Mar;25(2):213-8.2.J Exp Clin Cancer Res. 2009 Jul 25;28:106.
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$ 385.00
Cat# AO2090a
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