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KDM5B Antibody

Purified Mouse Monoclonal Antibody

  • E - KDM5B Antibody AO2094a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - KDM5B Antibody AO2094a

    Figure 2:Western blot analysis using KDM5B mAb against human KDM5B (AA: 231-319) recombinant protein. (Expected MW is 36.2 kDa)

  • WB - KDM5B Antibody AO2094a

    Figure 3:Western blot analysis using KDM5B mAb against HEK293 (1) and KDM5B (AA: 231-319)-hIgGFc transfected HEK293 (2) cell lysate.

  • FC - KDM5B Antibody AO2094a

    Figure 4:Flow cytometric analysis of MCF-7 cells using KDM5B mouse mAb (green) and negative control (red).

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9UGL1
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 7H3D7
Isotype IgG1
Calculated MW 175.6kDa
Description KDM5B (lysine (K)-specific demethylase 5B) is a protein-coding gene. Diseases associated with KDM5B include retinoblastoma. GO annotations related to this gene include oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors and sequence-specific DNA binding transcription factor activity. An important paralog of this gene is KDM5C.
Immunogen Purified recombinant fragment of human KDM5B (AA: 231-319) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 10765
Other Names Lysine-specific demethylase 5B, 1.14.11.-, Cancer/testis antigen 31, CT31, Histone demethylase JARID1B, Jumonji/ARID domain-containing protein 1B, PLU-1, Retinoblastoma-binding protein 2 homolog 1, RBP2-H1, KDM5B, JARID1B, PLU1, RBBP2H1
Dilution E~~1/10000
WB~~1/500 - 1/2000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsKDM5B Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name KDM5B
Synonyms JARID1B, PLU1, RBBP2H1
Function Histone demethylase that demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9' or H3 'Lys-27'. Demethylates trimethylated, dimethylated and monomethylated H3 'Lys-4'. Acts as a transcriptional corepressor for FOXG1B and PAX9. Favors the proliferation of breast cancer cells by repressing tumor suppressor genes such as BRCA1 and HOXA5. In contrast, may act as a tumor suppressor for melanoma. Represses the CLOCK-ARNTL/BMAL1 heterodimer-mediated transcriptional activation of the core clock component PER2 (By similarity).
Cellular Location Nucleus {ECO:0000255|PROSITE- ProRule:PRU00355, ECO:0000255|PROSITE-ProRule:PRU00537, ECO:0000269|PubMed:10336460, ECO:0000269|PubMed:12237901}
Tissue Location Ubiquitously expressed, with highest levels in testis. Down-regulated in melanoma and glioblastoma. Up-regulated in breast cancer (at protein level).
Citations (0)

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1.Cancer Cell. 2014 Jun 16;25(6):762-77.2.Int J Oncol. 2013 Apr;42(4):1212-8.

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$ 385.00
Cat# AO2094a
(40 western blots)
Availability: 7-10 days
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