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RND3 Antibody

Purified Mouse Monoclonal Antibody

  • E - RND3 Antibody AO2097a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - RND3 Antibody AO2097a

    Figure 2:Western blot analysis using RND3 mAb against human RND3 (AA: 104-241) recombinant protein. (Expected MW is 41.3 kDa)

  • WB - RND3 Antibody AO2097a

    Figure 3:Western blot analysis using RND3 mAb against HEK293 (1) and RND3 (AA: 104-241)-hIgGFc transfected HEK293 (2) cell lysate.

  • IF - RND3 Antibody AO2097a

    Figure 4:Immunofluorescence analysis of Hela cells using RND3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

  • FC - RND3 Antibody AO2097a

    Figure 5:Flow cytometric analysis of Hela cells using RND3 mouse mAb (green) and negative control (red).

  • IHC - RND3 Antibody AO2097a

    Figure 6:Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using RND3 mouse mAb with DAB staining.

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P61587
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 5C7B6
Isotype IgG1
Calculated MW 27.4kDa
Description This gene encodes a protein which is a member of the small GTPase protein superfamily. The encoded protein binds only GTP but has no GTPase activity, and appears to act as a negative regulator of cytoskeletal organization leading to loss of adhesion. Multiple alternatively spliced variants, encoding the same protein, have been identified.
Immunogen Purified recombinant fragment of human RND3 (AA: 104-241) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 390
Other Names Rho-related GTP-binding protein RhoE, Protein MemB, Rho family GTPase 3, Rho-related GTP-binding protein Rho8, Rnd3, RND3, ARHE, RHO8, RHOE
Dilution E~~1/10000
WB~~1/500 - 1/2000
IF~~1/200 - 1/1000
FC~~1/200 - 1/400
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRND3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RND3
Synonyms ARHE, RHO8, RHOE
Function Binds GTP but lacks intrinsic GTPase activity and is resistant to Rho-specific GTPase-activating proteins.
Cellular Location Golgi apparatus membrane; Peripheral membrane protein
Tissue Location Ubiquitous.
Research Areas
Citations (0)

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1.Hepatology. 2013 Jan;57(1):152-61. 2.Oncol Rep. 2011 Jan;25(1):173-80.

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$ 385.00
Cat# AO2097a
(40 western blots)
Availability: 7-10 days
Bulk Size
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