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PAX6 Antibody

Purified Mouse Monoclonal Antibody

  • E - PAX6 Antibody AO2103a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - PAX6 Antibody AO2103a

    Figure 2:Western blot analysis using PAX6 mAb against human PAX6 (AA: 1-122) recombinant protein. (Expected MW is 39.2 kDa)

  • WB - PAX6 Antibody AO2103a

    Figure 3:Western blot analysis using PAX6 mAb against HEK293 (1) and PAX6 (AA: 1-122)-hIgGFc transfected HEK293 (2) cell lysate.

  • FC - PAX6 Antibody AO2103a

    Figure 4:Flow cytometric analysis of A549 cells using PAX6 mouse mAb (green) and negative control (red).

  • IHC - PAX6 Antibody AO2103a

    Figure 5:Immunohistochemical analysis of paraffin-embedded colon cancer tissues using PAX6 mouse mAb with DAB staining.

  • IHC - PAX6 Antibody AO2103a

    Figure 6:Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using PAX6 mouse mAb with DAB staining.

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P26367
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 1F6G2
Isotype IgG1
Calculated MW 46.7kDa
Description This gene encodes paired box gene 6, one of many human homologs of the Drosophila melanogaster gene prd. In addition to the hallmark feature of this gene family, a conserved paired box domain, the encoded protein also contains a homeo box domain. Both domains are known to bind DNA and function as regulators of gene transcription. This gene is expressed in the developing nervous system, and in developing eyes. Mutations in this gene are known to cause ocular disorders such as aniridia and Peter's anomaly. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Immunogen Purified recombinant fragment of human PAX6 (AA: 1-122) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 5080
Other Names Paired box protein Pax-6, Aniridia type II protein, Oculorhombin, PAX6, AN2
Dilution E~~1/10000
WB~~1/500 - 1/2000
FC~~1/200 - 1/400
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPAX6 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PAX6
Synonyms AN2
Function Transcription factor with important functions in the development of the eye, nose, central nervous system and pancreas. Required for the differentiation of pancreatic islet alpha cells (By similarity). Competes with PAX4 in binding to a common element in the glucagon, insulin and somatostatin promoters. Regulates specification of the ventral neuron subtypes by establishing the correct progenitor domains (By similarity). Isoform 5a appears to function as a molecular switch that specifies target genes.
Cellular Location Nucleus.
Tissue Location Fetal eye, brain, spinal cord and olfactory epithelium. Isoform 5a is less abundant than the PAX6 shorter form
Research Areas
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1.PLoS One. 2014 Jan 15;9(1):e85738.2.Curr Eye Res. 2012 Oct;37(10):879-83.

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$ 385.00
Cat# AO2103a
(40 western blots)
Availability: 7-10 days
Bulk Size
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