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ATF3 Antibody

Purified Mouse Monoclonal Antibody

  • E - ATF3 Antibody AO2104a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - ATF3 Antibody AO2104a

    Figure 2:Western blot analysis using ATF3 mAb against human ATF3 (AA: 1-181) recombinant protein. (Expected MW is *** kDa)

  • WB - ATF3 Antibody AO2104a

    Figure 3:Western blot analysis using ATF3 mAb against HEK293 (1) and ATF3 (AA: 1-181)-hIgGFc transfected HEK293 (2) cell lysate.

  • FC - ATF3 Antibody AO2104a

    Figure 5:Flow cytometric analysis of A549 cells using ATF3 mouse mAb (green) and negative control (red).

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P18847
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 5A9B9
Isotype IgG1
Calculated MW 20.6kDa
Description This gene encodes a member of the mammalian activation transcription factor/cAMP responsive element-binding (CREB) protein family of transcription factors. This gene is induced by a variety of signals, including many of those encountered by cancer cells, and is involved in the complex process of cellular stress response. Multiple transcript variants encoding different isoforms have been found for this gene. It is possible that alternative splicing of this gene may be physiologically important in the regulation of target genes.
Immunogen Purified recombinant fragment of human ATF3 (AA: 1-181) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 467
Other Names Cyclic AMP-dependent transcription factor ATF-3, cAMP-dependent transcription factor ATF-3, Activating transcription factor 3, ATF3
Dilution E~~1/10000
WB~~1/500 - 1/2000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsATF3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ATF3
Function This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Represses transcription from promoters with ATF sites. It may repress transcription by stabilizing the binding of inhibitory cofactors at the promoter. Isoform 2 activates transcription presumably by sequestering inhibitory cofactors away from the promoters.
Cellular Location Nucleus {ECO:0000255|PROSITE- ProRule:PRU00978, ECO:0000269|PubMed:12034827}
Research Areas
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1.Arch Biochem Biophys. 2014 Dec 15;564:203-10.2.Tumour Biol. 2014 Aug;35(8):8329-34.

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$ 385.00
Cat# AO2104a
(40 western blots)
Availability: 7-10 days
Bulk Size
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