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AOF1 Antibody

Purified Mouse Monoclonal Antibody

  • E - AOF1 Antibody AO2105a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - AOF1 Antibody AO2105a

    Figure 2:Western blot analysis using AOF1 mAb against human AOF1 (AA: 6-129) recombinant protein. (Expected MW is 40 kDa)

  • WB - AOF1 Antibody AO2105a

    Figure 3:Western blot analysis using AOF1 mAb against HEK293 (1) and AOF1 (AA: 6-129)-hIgGFc transfected HEK293 (2) cell lysate.

  • IF - AOF1 Antibody AO2105a

    Figure 4:Immunofluorescence analysis of HeLa cells using AOF1 mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

  • IF - AOF1 Antibody AO2105a

    Figure 5:Immunofluorescence analysis of HeLa cells using AOF1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

  • IHC - AOF1 Antibody AO2105a

    Figure 6:Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using AOF1 mouse mAb with DAB staining.

  • IHC - AOF1 Antibody AO2105a

    Figure 7:Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using AOF1 mouse mAb with DAB staining.

  • FC - AOF1 Antibody AO2105a

    Figure 8:Flow cytometric analysis of HeLa cells using AOF1 mouse mAb (green) and negative control (red).

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q8NB78
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 5E10C1
Isotype IgG1
Calculated MW 92kDa
Description Flavin-dependent histone demethylases, such as KDM1B, regulate histone lysine methylation, an epigenetic mark that regulates gene expression and chromatin function.
Immunogen Purified recombinant fragment of human AOF1 (AA: 6-129) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 221656
Other Names Lysine-specific histone demethylase 1B, 1.-.-.-, Flavin-containing amine oxidase domain-containing protein 1, Lysine-specific histone demethylase 2, KDM1B, AOF1, C6orf193, LSD2
Dilution E~~1/10000
WB~~1/500 - 1/2000
IF~~1/200 - 1/1000
IHC~~1/200 - 1/1000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsAOF1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name KDM1B
Synonyms AOF1, C6orf193, LSD2
Function Histone demethylase that demethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. Required for de novo DNA methylation of a subset of imprinted genes during oogenesis. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Demethylates both mono- and di-methylated 'Lys-4' of histone H3. Has no effect on tri- methylated 'Lys-4', mono-, di- or tri-methylated 'Lys-9', mono-, di- or tri-methylated 'Lys-27', mono-, di- or tri-methylated 'Lys- 36' of histone H3, or on mono-, di- or tri-methylated 'Lys-20' of histone H4 (By similarity).
Cellular Location Nucleus.
Citations (0)

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1.Cell Res. 2013 Feb;23(2):225-41.2.Nature. 2009 Sep 17;461(7262):415-8.

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$ 385.00
Cat# AO2105a
(40 western blots)
Availability: 7-10 days
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