|Application ||WB, IHC, FC, ICC, E|
|Description||Flavin-dependent histone demethylases, such as KDM1B, regulate histone lysine methylation, an epigenetic mark that regulates gene expression and chromatin function.|
|Immunogen||Purified recombinant fragment of human AOF1 (AA: 6-129) expressed in E. Coli.|
|Formulation||Purified antibody in PBS with 0.05% sodium azide|
|Other Names||Lysine-specific histone demethylase 1B, 1.-.-.-, Flavin-containing amine oxidase domain-containing protein 1, Lysine-specific histone demethylase 2, KDM1B, AOF1, C6orf193, LSD2|
WB~~1/500 - 1/2000
IF~~1/200 - 1/1000
IHC~~1/200 - 1/1000
FC~~1/200 - 1/400
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||AOF1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||AOF1, C6orf193, LSD2|
|Function||Histone demethylase that demethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. Required for de novo DNA methylation of a subset of imprinted genes during oogenesis. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Demethylates both mono- and di-methylated 'Lys-4' of histone H3. Has no effect on tri- methylated 'Lys-4', mono-, di- or tri-methylated 'Lys-9', mono-, di- or tri-methylated 'Lys-27', mono-, di- or tri-methylated 'Lys- 36' of histone H3, or on mono-, di- or tri-methylated 'Lys-20' of histone H4 (By similarity).|
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Provided below are standard protocols that you may find useful for product applications.
1.Cell Res. 2013 Feb;23(2):225-41.2.Nature. 2009 Sep 17;461(7262):415-8.
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