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AOF1 Antibody
Purified Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC, FC, ICC, E |
|---|---|
| Primary Accession | Q8NB78 |
| Reactivity | Human |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone Names | 5E10C1 |
| Isotype | IgG1 |
| Calculated MW | 92kDa |
| Description | Flavin-dependent histone demethylases, such as KDM1B, regulate histone lysine methylation, an epigenetic mark that regulates gene expression and chromatin function. |
| Immunogen | Purified recombinant fragment of human AOF1 (AA: 6-129) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
| Gene ID | 221656 |
|---|---|
| Other Names | Lysine-specific histone demethylase 1B, 1.-.-.-, Flavin-containing amine oxidase domain-containing protein 1, Lysine-specific histone demethylase 2, KDM1B, AOF1, C6orf193, LSD2 |
| Dilution | E~~1/10000 WB~~1/500 - 1/2000 IF~~1/200 - 1/1000 IHC~~1/200 - 1/1000 FC~~1/200 - 1/400 |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | AOF1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | KDM1B (HGNC:21577) |
|---|---|
| Function | Histone demethylase that demethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. Required for de novo DNA methylation of a subset of imprinted genes during oogenesis. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Demethylates both mono- and di-methylated 'Lys-4' of histone H3. Has no effect on tri-methylated 'Lys-4', mono-, di- or tri-methylated 'Lys-9', mono-, di- or tri-methylated 'Lys-27', mono-, di- or tri-methylated 'Lys-36' of histone H3, or on mono-, di- or tri-methylated 'Lys-20' of histone H4. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of GLYR1 to achieve such activity, they form a multifunctional enzyme complex that modifies transcribed chromatin and facilitates Pol II transcription through nucleosomes (PubMed:30970244). |
| Cellular Location | Nucleus. Chromosome. Note=Found in actively RNAPolII- transcribed gene bodies. |
Citations (0)
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Application Protocols
Provided below are standard protocols that you may find useful for product applications.
References
1.Cell Res. 2013 Feb;23(2):225-41.2.Nature. 2009 Sep 17;461(7262):415-8.
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$ 385.00
Cat# AO2105a
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