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ATG16L1 Antibody

Purified Mouse Monoclonal Antibody

  • E - ATG16L1 Antibody AO2106a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - ATG16L1 Antibody AO2106a

    Figure 2:Western blot analysis using ATG16L1 mAb against human ATG16L1 (AA: 11-257) recombinant protein. (Expected MW is 55.8 kDa)

  • WB - ATG16L1 Antibody AO2106a

    Figure 3:Western blot analysis using ATG16L1 mAb against HEK293 (1) and ATG16L1 (AA: 11-257)-hIgGFc transfected HEK293 (2) cell lysate.

  • WB - ATG16L1 Antibody AO2106a

    Figure 4:Western blot analysis using ATG16L1 mouse mAb against Hela (1) cell lysate.

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q676U5
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 8F8C2
Isotype IgG1
Calculated MW 68.3kDa
Description The protein encoded by this gene is part of a large protein complex that is necessary for autophagy, the major process by which intracellular components are targeted to lysosomes for degradation. Defects in this gene are a cause of susceptibility to inflammatory bowel disease type 10 (IBD10). Several transcript variants encoding different isoforms have been found for this gene.
Immunogen Purified recombinant fragment of human ATG16L1 (AA: 11-257) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 55054
Other Names Autophagy-related protein 16-1, APG16-like 1, ATG16L1, APG16L
Dilution E~~1/10000
WB~~1/500 - 1/2000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsATG16L1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ATG16L1
Synonyms APG16L
Function Plays an essential role in autophagy: interacts with ATG12-ATG5 to mediate the conjugation of phosphatidylethanolamine (PE) to LC3 (MAP1LC3A, MAP1LC3B or MAP1LC3C), to produce a membrane-bound activated form of LC3 named LC3-II. Thereby, controls the elongation of the nascent autophagosomal membrane.
Cellular Location Cytoplasm. Preautophagosomal structure membrane; Peripheral membrane protein. Note=Recruited to omegasomes membranes by WIPI2 Omegasomes are endoplasmic reticulum connected strutures at the origin of preautophagosomal structures. Localized to preautophagosomal structure (PAS) where it is involved in the membrane targeting of ATG5. Localizes also to discrete punctae along the ciliary axoneme. {ECO:0000250|UniProtKB:Q8C0J2}
Research Areas
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1.Autophagy. 2012 Sep;8(9):1387-8.2.Inflamm Bowel Dis. 2011 Jul;17(7):1635-6.

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$ 385.00
Cat# AO2106a
(40 western blots)
Availability: 7-10 days
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