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PMS2 Antibody

Purified Mouse Monoclonal Antibody

  • E - PMS2 Antibody AO2121a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - PMS2 Antibody AO2121a

    Figure 2:Western blot analysis using PMS2 mAb against human PMS2 (AA: 748-851) recombinant protein. (Expected MW is 37.7 kDa)

  • WB - PMS2 Antibody AO2121a

    Figure 3:Western blot analysis using PMS2 mAb against HEK293 (1) and PMS2 (AA: 748-851)-hIgGFc transfected HEK293 (2) cell lysate.

  • IF - PMS2 Antibody AO2121a

    Figure 4:Immunofluorescence analysis of HeLa cells using PMS2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

  • IF - PMS2 Antibody AO2121a

    Figure 5:Immunofluorescence analysis of MCF-7 cells using PMS2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

  • FC - PMS2 Antibody AO2121a

    Figure 6:Flow cytometric analysis of HeLa cells using PMS2 mouse mAb (green) and negative control (red).

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P54278
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 1E9D11
Isotype IgG1
Calculated MW 95.8kDa
Description This gene is one of the PMS2 gene family members found in clusters on chromosome 7. The product of this gene is involved in DNA mismatch repair. It forms a heterodimer with MLH1 and this complex interacts with other complexes bound to mismatched bases. Mutations in this gene are associated with hereditary nonpolyposis colorectal cancer, Turcot syndrome, and are a cause of supratentorial primitive neuroectodermal tumors. Alternatively spliced transcript variants have been observed for this gene.
Immunogen Purified recombinant fragment of human PMS2 (AA: 748-851) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 5395
Other Names Mismatch repair endonuclease PMS2, 3.1.-.-, DNA mismatch repair protein PMS2, PMS1 protein homolog 2, PMS2, PMSL2
Dilution E~~1/10000
WB~~1/500 - 1/2000
IF~~1/200 - 1/1000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPMS2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PMS2
Synonyms PMSL2
Function Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MLH1 to form MutL alpha. DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2- MSH6) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages.
Cellular Location Nucleus.
Citations (0)

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1.J Med Genet. 2013 Aug;50(8):552-63.2.Hum Mutat. 2010 May;31(5):552-60.

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$ 385.00
Cat# AO2121a
(40 western blots)
Availability: 7-10 days
Bulk Size
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