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CK5 Antibody

Purified Mouse Monoclonal Antibody

  • E - CK5 Antibody AO2150a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - CK5 Antibody AO2150a

    Figure 2:Western blot analysis using CK5 mAb against human CK5 (AA: 258-357) recombinant protein. (Expected MW is 33.1 kDa)

  • WB - CK5 Antibody AO2150a

    Figure 3:Western blot analysis using CK5 mAb against HEK293 (1) and CK5 (AA: 258-357)-hIgGFc transfected HEK293 (2) cell lysate.

  • WB - CK5 Antibody AO2150a

    Figure 4:Western blot analysis using CK5 mouse mAb against A431 (1), 3T3-L1 (2), COS7 (3), MCF-7 (4), SK-Br-3 (5), Hela (6), Lncap (7), and HepG2 (8) cell lysate.

  • IF - CK5 Antibody AO2150a

    Figure 5:Immunofluorescence analysis of MCF-7 cells using CK5 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

  • IHC - CK5 Antibody AO2150a

    Figure 6:Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using CK5 mouse mAb with DAB staining.

  • FC - CK5 Antibody AO2150a

    Figure 7:Flow cytometric analysis of HeLa cells using CK5 mouse mAb (green) and negative control (red).

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P13647
Reactivity Human, Mouse, Monkey
Host Mouse
Clonality Monoclonal
Clone Names 3D6D4
Isotype IgG1
Calculated MW 62.4kDa
Description The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13.
Immunogen Purified recombinant fragment of human CK5 (AA: 258-357) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 3852
Other Names Keratin, type II cytoskeletal 5, 58 kDa cytokeratin, Cytokeratin-5, CK-5, Keratin-5, K5, Type-II keratin Kb5, KRT5
Dilution E~~1/10000
WB~~1/500 - 1/2000
IF~~1/200 - 1/1000
IHC~~1/200 - 1/1000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCK5 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name KRT5
Research Areas
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1.Horm Cancer. 2013 Feb;4(1):36-49.2.Breast Cancer Res Treat. 2011 Jul;128(1):45-55.

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$ 385.00
Cat# AO2150a
(40 western blots)
Availability: 7-10 days
Bulk Size
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