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SIRT4 Antibody

Purified Mouse Monoclonal Antibody

  • E - SIRT4 Antibody AO2152a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - SIRT4 Antibody AO2152a

    Figure 2:Western blot analysis using SIRT4 mAb against human SIRT4 (AA: 215-314) recombinant protein. (Expected MW is 37 kDa)

  • WB - SIRT4 Antibody AO2152a

    Figure 3:Western blot analysis using SIRT4 mAb against HEK293 (1) and SIRT4 (AA: 215-314)-hIgGFc transfected HEK293 (2) cell lysate.

  • IF - SIRT4 Antibody AO2152a

    Figure 5:Immunofluorescence analysis of HeLa cells using SIRT4 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

  • FC - SIRT4 Antibody AO2152a

    Figure 5:Flow cytometric analysis of HeLa cells using SIRT4 mouse mAb (green) and negative control (red).

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9Y6E7
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 6A12D7
Isotype IgG1
Calculated MW 35.2kDa
Description This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class IV of the sirtuin family.
Immunogen Purified recombinant fragment of human SIRT4 (AA: 215-314) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 23409
Other Names NAD-dependent protein deacetylase sirtuin-4 {ECO:0000255|HAMAP-Rule:MF_03161}, 3.5.1.- {ECO:0000255|HAMAP-Rule:MF_03161}, NAD-dependent ADP-ribosyltransferase sirtuin-4 {ECO:0000255|HAMAP-Rule:MF_03161}, 2.4.2.- {ECO:0000255|HAMAP-Rule:MF_03161}, Regulatory protein SIR2 homolog 4 {ECO:0000255|HAMAP-Rule:MF_03161}, SIR2-like protein 4 {ECO:0000255|HAMAP-Rule:MF_03161}, SIRT4 {ECO:0000255|HAMAP-Rule:MF_03161}, SIR2L4
Dilution E~~1/10000
WB~~1/500 - 1/2000
IF~~1/200 - 1/1000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSIRT4 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SIRT4 {ECO:0000255|HAMAP-Rule:MF_03161}
Synonyms SIR2L4
Function Acts both as NAD-dependent protein ADP-ribosyl transferase and NAD-dependent protein deacetylase. Catalyzes the transfer of ADP-ribosyl groups onto target proteins, including mitochondrial GLUD1, inhibiting GLUD1 enzyme activity. Acts as a negative regulator of mitochondrial glutamine metabolism by mediating mono ADP-ribosylation of GLUD1: expressed in response to DNA damage and negatively regulates anaplerosis by inhibiting GLUD1, leading to block metabolism of glutamine into tricarboxylic acid cycle and promoting cell cycle arrest. In response to mTORC1 signal, SIRT4 expression is repressed, promoting anaplerosis and cell proliferation. Acts as a tumor suppressor. Also acts as a NAD-dependent protein deacetylase: mediates deacetylation of 'Lys- 471' of MLYCD, inhibiting its activity, thereby acting as a regulator of lipid homeostasis. Down-regulates insulin secretion.
Cellular Location Mitochondrion matrix {ECO:0000255|HAMAP- Rule:MF_03161, ECO:0000269|PubMed:16079181, ECO:0000269|PubMed:16959573, ECO:0000269|PubMed:17715127}
Tissue Location Detected in vascular smooth muscle and striated muscle. Detected in insulin-producing beta-cells in pancreas islets of Langerhans (at protein level). Widely expressed. Weakly expressed in leukocytes and fetal thymus
Research Areas
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1.Cell. 2014 Dec 18;159(7):1615-25.2.Mol Cell Biol. 2013 Nov;33(22):4552-61.

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$ 385.00
Cat# AO2152a
(40 western blots)
Availability: 7-10 days
Bulk Size
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