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RAD52 Antibody

Purified Mouse Monoclonal Antibody

  • E - RAD52 Antibody AO2154a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - RAD52 Antibody AO2154a

    Figure 2:Western blot analysis using RAD52 mAb against human RAD52 (AA: 269-418) recombinant protein. (Expected MW is 42.5 kDa)

  • WB - RAD52 Antibody AO2154a

    Figure 3:Western blot analysis using RAD52 mAb against HEK293 (1) and RAD52 (AA: 269-418)-hIgGFc transfected HEK293 (2) cell lysate.

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P43351
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 5E11B2
Isotype IgG1
Calculated MW 46.2kDa
Description The protein encoded by this gene shares similarity with Saccharomyces cerevisiae Rad52, a protein important for DNA double-strand break repair and homologous recombination. This gene product was shown to bind single-stranded DNA ends, and mediate the DNA-DNA interaction necessary for the annealing of complementary DNA strands. It was also found to interact with DNA recombination protein RAD51, which suggested its role in RAD51 related DNA recombination and repair. A pseudogene of this gene is present on chromosome 2. Alternative splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known.
Immunogen Purified recombinant fragment of human RAD52 (AA: 269-418) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 5893
Other Names DNA repair protein RAD52 homolog, RAD52
Dilution E~~1/10000
WB~~1/500 - 1/2000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRAD52 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RAD52
Function Involved in double-stranded break repair. Plays a central role in genetic recombination and DNA repair by promoting the annealing of complementary single-stranded DNA and by stimulation of the RAD51 recombinase.
Cellular Location Nucleus.
Citations (0)

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1.Blood. 2013 Aug 15;122(7):1293-304. 2.PLoS One. 2012;7(11):e50461.

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$ 385.00
Cat# AO2154a
(40 western blots)
Availability: 7-10 days
Bulk Size
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