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WNT3A Antibody

Purified Mouse Monoclonal Antibody

  • E - WNT3A Antibody AO2160a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - WNT3A Antibody AO2160a

    Figure 2:Western blot analysis using WNT3A mAb against human WNT3A (AA: 170-352) recombinant protein. (Expected MW is 47.1 kDa)

  • WB - WNT3A Antibody AO2160a

    Figure 3:Western blot analysis using WNT3A mAb against HEK293 (1) and WNT3A (AA: 170-352)-hIgGFc transfected HEK293 (2) cell lysate.

  • WB - WNT3A Antibody AO2160a

    Figure 4:Western blot analysis using WNT3A mouse mAb against A549 (1), HepG2 (2), and A431 (3) cell lysate.

  • IF - WNT3A Antibody AO2160a

    Figure 5:Immunofluorescence analysis of HeLa cells using WNT3A mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

  • FC - WNT3A Antibody AO2160a

    Figure 6:Flow cytometric analysis of HeLa cells using WNT3A mouse mAb (green) and negative control (red).

  • IHC - WNT3A Antibody AO2160a

    Figure 7:Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using WNT3A mouse mAb with DAB staining.

  • IHC - WNT3A Antibody AO2160a

    Figure 8:Immunohistochemical analysis of paraffin-embedded endometrial cancer tissues using WNT3A mouse mAb with DAB staining.

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P56704
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 1E6G4
Isotype IgG2b
Calculated MW 39.4kDa
Description The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 96% amino acid identity to mouse Wnt3A protein, and 84% to human WNT3 protein, another WNT gene product. This gene is clustered with WNT14 gene, another family member, in chromosome 1q42 region.
Immunogen Purified recombinant fragment of human WNT3A (AA: 170-352) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 89780
Other Names Protein Wnt-3a, WNT3A
Dilution E~~1/10000
WB~~1/500 - 1/2000
IF~~1/200 - 1/1000
FC~~1/200 - 1/400
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsWNT3A Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name WNT3A
Function Ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube.
Cellular Location Secreted, extracellular space, extracellular matrix
Tissue Location Moderately expressed in placenta and at low levels in adult lung, spleen, and prostate
Research Areas
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1.Mol Cell Neurosci. 2013 May;54:44-57.2.J Dermatol Sci. 2011 Dec;64(3):199-209.

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$ 385.00
Cat# AO2160a
(40 western blots)
Availability: 7-10 days
Bulk Size
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